Foundations in Microbiology
9th Edition
ISBN: 9780073522609
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 7.L1, Problem 1WC
Summary Introduction
Introduction:
The process of movement of water molecules along the gradient across cell membrane is known as osmosis. The water molecules move from a region of higher concentration to a region of low concentration. Depending on the solute concentration, a solution may be hypotonic, hypertonic or isotonic.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 7 Solutions
Foundations in Microbiology
Ch. 7.1 - 1. Describe the major environmental factors to...Ch. 7.1 - 2. Define nutrition and nutrients and their...Ch. 7.1 - 3. Differentiate between organic and inorganic...Ch. 7.1 - Prob. 4ELOCh. 7.1 - Prob. 1CYPCh. 7.1 - Prob. 2CYPCh. 7.1 - 3. List the general functions of the essential...Ch. 7.1 - 4. Define growth factors and metallic ions with...Ch. 7.2 - 5. Describe the main categories of nutritional...Ch. 7.2 - 6. Distinguish different types of autotrophs and...
Ch. 7.2 - Prob. 7ELOCh. 7.2 - 5. Compare autotrophs and heterotrophs with...Ch. 7.2 - 6. Describe the nutritional strategy of two types...Ch. 7.2 - Prob. 7CYPCh. 7.2 - Prob. 8CYPCh. 7.3 - 8. Describe the basic factors in diffusion and...Ch. 7.3 - Prob. 9ELOCh. 7.3 - 10. Analyze adaptations microbes make in response...Ch. 7.3 - Prob. 11ELOCh. 7.3 - 9. Compare and contrast passive and active forms...Ch. 7.3 - Prob. 10CYPCh. 7.3 - 11. Explain the differences between facilitated...Ch. 7.3 - 12. Compare the effects of isotonic, hypotonic,...Ch. 7.4 - 12. Differentiate between habitat and niche.Ch. 7.4 - 13. Describe the range of temperatures a microbe...Ch. 7.4 - Prob. 14ELOCh. 7.4 - Prob. 15ELOCh. 7.4 - Prob. 16ELOCh. 7.4 - Prob. 17ELOCh. 7.4 - Prob. 13CYPCh. 7.4 - Prob. 14CYPCh. 7.4 - 15. Explain what it means to be an obligate...Ch. 7.4 - 16. Where in the body are anaerobic habitats apt...Ch. 7.4 - Prob. 17CYPCh. 7.5 - 18. Outline the types of associations among...Ch. 7.5 - Prob. 19ELOCh. 7.5 - Prob. 20ELOCh. 7.5 - Prob. 21ELOCh. 7.5 - Prob. 22ELOCh. 7.5 - Prob. 18CYPCh. 7.5 - Prob. 19CYPCh. 7.5 - Prob. 20CYPCh. 7.5 - 21. Relate several advantages to communication...Ch. 7.6 - Prob. 23ELOCh. 7.6 - 24. Describe the process of population growth and...Ch. 7.6 - 25. Explain the stages in the population growth...Ch. 7.6 - Prob. 26ELOCh. 7.6 - 22. What is microbial growth? Explain the stages...Ch. 7.6 - 23. Why is growth called exponential? What causes...Ch. 7.6 - 24. Contrast two different methods of detecting...Ch. 7.6 - 25. Explain the relationship between colony counts...Ch. 7.L1 - 1. An organic nutrient essential to an...Ch. 7.L1 - Prob. 2MCQCh. 7.L1 - 3. An organism that can synthesize all its...Ch. 7.L1 - Prob. 4MCQCh. 7.L1 - Prob. 5MCQCh. 7.L1 - 6. Which of the following substances are required...Ch. 7.L1 - 7. A pathogen would most accurately be described...Ch. 7.L1 - Prob. 8MCQCh. 7.L1 - Prob. 9MCQCh. 7.L1 - Prob. 10MCQCh. 7.L1 - Prob. 11MCQCh. 7.L1 - 12. Which of the following is not involved in...Ch. 7.L1 - 13. Superoxide ion is toxic to strict anaerobes...Ch. 7.L1 - Prob. 14MCQCh. 7.L1 - 15. In a viable plate count, each ____ represents...Ch. 7.L1 - 16. The stage in population growth with the...Ch. 7.L1 - Prob. 1CSRCh. 7.L1 - Prob. 2CSRCh. 7.L1 - Prob. 3CSRCh. 7.L1 - Prob. 1WCCh. 7.L1 - Prob. 2WCCh. 7.L1 - Prob. 3WCCh. 7.L1 - Prob. 4WCCh. 7.L1 - 5. a. What biochemical events in quorum sensing...Ch. 7.L1 - 6. Explain what is happening to the population at...Ch. 7.L1 - Prob. 7WCCh. 7.L2 - 1. a. Is there a microbe that could grow on a...Ch. 7.L2 - 2. Describe how one might determine the nutrient...Ch. 7.L2 - 3. Patients with ketoacidosis associated with...Ch. 7.L2 - Prob. 4CTCh. 7.L2 - 5. Provide some suggestions for treating anaerobic...Ch. 7.L2 - Prob. 6CTCh. 7.L2 - Prob. 7CTCh. 7.L2 - Prob. 8CTCh. 7.L2 - 9. Describe the similarities and differences...Ch. 7.L2 - 1. Place appropriate points on the axes and draw...Ch. 7.L2 - Prob. 2VC
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
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