GENETICS:ANALYSIS+PRIN.(LL)-W/ACCESS
GENETICS:ANALYSIS+PRIN.(LL)-W/ACCESS
6th Edition
ISBN: 9781260239775
Author: BROOKER
Publisher: MCG
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Chapter 7, Problem 7EQ

As mentioned in question 2 of More Genetic TIPS, origins of transfer can be located in many different locations, and their direction of transfer can be clockwise or counterclockwise. Let’s suppose a researcher conjugated six different Hfr strains that were t h r + l e u + t o n s s t r r a z i s l a c + g a l + p r o + m e t + to an F strain that was t h r l e u t o n r s t r s a z i r l a c g a l p r o m e t , and obtained the following results:

Strain Order of Gene Transfer
1 t o n s a z i s l e u + t h r + m e t + s t r r g a l + l a c + p r o +
2 l e u + a z i s t o n s p r o + l a c + g a l + s t r r m e t + t h r +
3 l a c + g a l + s t r r m e t + t h r + l e u + a z i s t o n s p r o +
4 l e u + t h r + m e t + s t r r g a l + l a c + p r o + t o n s a z i s
5 t o n s   p r o +   l a c +   g a l + s t r r m e t + t h r + l e u + a z i s
6 m e t + s t r r g a l + l a c + p r o + t o n s a z i s l e u + t h r +

Draw a circular map of the E. coli chromosome that shows the locations and orientations of the origins of transfer in these six Hfr strains.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

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GENETICS:ANALYSIS+PRIN.(LL)-W/ACCESS

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