Genetics: Analysis and Principles
Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 7EQ

As mentioned in question 2 of More Genetic TIPS, origins of transfer can be located in many different locations, and their direction of transfer can be clockwise or counterclockwise. Let’s suppose a researcher conjugated six different Hfr strains that were t h r + l e u + t o n s s t r r a z i s l a c + g a l + p r o + m e t + to an F strain that was t h r l e u t o n r s t r s a z i r l a c g a l p r o m e t , and obtained the following results:

Strain Order of Gene Transfer
1 t o n s a z i s l e u + t h r + m e t + s t r r g a l + l a c + p r o +
2 l e u + a z i s t o n s p r o + l a c + g a l + s t r r m e t + t h r +
3 l a c + g a l + s t r r m e t + t h r + l e u + a z i s t o n s p r o +
4 l e u + t h r + m e t + s t r r g a l + l a c + p r o + t o n s a z i s
5 t o n s   p r o +   l a c +   g a l + s t r r m e t + t h r + l e u + a z i s
6 m e t + s t r r g a l + l a c + p r o + t o n s a z i s l e u + t h r +

Draw a circular map of the E. coli chromosome that shows the locations and orientations of the origins of transfer in these six Hfr strains.

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