BIOLOGY 12TH ED (LL) W/ CONNECT
BIOLOGY 12TH ED (LL) W/ CONNECT
12th Edition
ISBN: 9781264898091
Author: Raven
Publisher: MCG
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Chapter 7, Problem 7A
Summary Introduction

Saccharomyces cerevisiae, commonly known as yeast, is a eukaryotic organism that belongs to kingdom fungi. It is a single-celled microscopic organism. It is majorly used for fermentation processes post glycolysis, which leads to the production of ethanol along with carbon dioxide in anaerobic conditions.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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