HUMAN ANATOMY
6th Edition
ISBN: 9781260986037
Author: SALADIN
Publisher: MCG
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Chapter 7, Problem 6TYR
Summary Introduction
Introduction:
Intramembranous ossification is the process by which the mesenchymal tissue is converted into bone. Ossification begins as mesenchymal cells and differentiates into osteoblasts at the ossification center.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 7 Solutions
HUMAN ANATOMY
Ch. 7.1 - Name the major components of the axial skeleton....Ch. 7.1 - Explaine why an adult does not have as many bones...Ch. 7.1 - Briefly describe each of the following bone...Ch. 7.2 - Name the paranasal sinuses and state their...Ch. 7.2 - Prob. 5BYGOCh. 7.2 - Draw an oval representing a superior view of the...Ch. 7.2 - Prob. 7BYGOCh. 7.2 - Palpate as many of the following structures as...Ch. 7.3 - The lower we look on the vertebral column, the...Ch. 7.3 - How would head movements be affected if vertebrae...
Ch. 7.3 - Discuss the contributions of the intervertebral...Ch. 7.3 - Make a table with three columns headed cervical,...Ch. 7.3 - Prob. 11BYGOCh. 7.3 - Prob. 12BYGOCh. 7.3 - Distinguish between true, false, and floating...Ch. 7.3 - Palpate as many of the following structures as...Ch. 7.4 - Suppose you were studying a skull with some teeth...Ch. 7.4 - Prob. 2AWYKCh. 7.4 - Prob. 15BYGOCh. 7.4 - Prob. 16BYGOCh. 7.4 - Prob. 17BYGOCh. 7.4 - Prob. 18BYGOCh. 7.4 - Prob. 19BYGOCh. 7 - The distinction between the axial and appendicular...Ch. 7 - Prob. 7.1.2AYLOCh. 7 - Prob. 7.1.3AYLOCh. 7 - Prob. 7.2.1AYLOCh. 7 - Prob. 7.2.2AYLOCh. 7 - Prob. 7.2.3AYLOCh. 7 - Prob. 7.2.4AYLOCh. 7 - Prob. 7.2.5AYLOCh. 7 - Prob. 7.2.6AYLOCh. 7 - Prob. 7.2.7AYLOCh. 7 - Prob. 7.2.8AYLOCh. 7 - Recongnition of the important anatenucal features...Ch. 7 - The location, anatomy, and function of the hyoid...Ch. 7 - The names, locations and anatomy of the three...Ch. 7 - Prob. 7.2.12AYLOCh. 7 - Prob. 7.3.1AYLOCh. 7 - The five classes of vertebrae; and the number of...Ch. 7 - Prob. 7.3.3AYLOCh. 7 - Prob. 7.3.4AYLOCh. 7 - Prob. 7.3.5AYLOCh. 7 - Prob. 7.3.6AYLOCh. 7 - Prob. 7.3.7AYLOCh. 7 - Prob. 7.3.8AYLOCh. 7 - Prob. 7.3.9AYLOCh. 7 - Prob. 7.3.10AYLOCh. 7 - Prob. 7.3.11AYLOCh. 7 - Components of the thoracic cageCh. 7 - Prob. 7.3.13AYLOCh. 7 - Prob. 7.3.14AYLOCh. 7 - Prob. 7.3.15AYLOCh. 7 - Prob. 7.4.1AYLOCh. 7 - Prob. 7.4.2AYLOCh. 7 - The role of pharyngeal arches in development of...Ch. 7 - Prob. 7.4.4AYLOCh. 7 - Prob. 7.4.5AYLOCh. 7 - Prob. 7.4.6AYLOCh. 7 - Prob. 7.4.7AYLOCh. 7 - Prob. 7.4.8AYLOCh. 7 - Prob. 7.4.9AYLOCh. 7 - Prob. 7.4.10AYLOCh. 7 - Prob. 7.4.11AYLOCh. 7 - Prob. 7.4.12AYLOCh. 7 - Prob. 1TYRCh. 7 - Prob. 2TYRCh. 7 - Prob. 3TYRCh. 7 - Prob. 4TYRCh. 7 - Thoracic vertebrae do not have transverse...Ch. 7 - Prob. 6TYRCh. 7 - Prob. 7TYRCh. 7 - Prob. 8TYRCh. 7 - Prob. 9TYRCh. 7 - Prob. 10TYRCh. 7 - Prob. 11TYRCh. 7 - Prob. 12TYRCh. 7 - Prob. 13TYRCh. 7 - Prob. 14TYRCh. 7 - Prob. 15TYRCh. 7 - Prob. 16TYRCh. 7 - Prob. 17TYRCh. 7 - We have five pairs of _________ ribs and two pairs...Ch. 7 - Prob. 19TYRCh. 7 - The point at the inferior end of the sterminum is...Ch. 7 - Prob. 1BYMVCh. 7 - Prob. 2BYMVCh. 7 - Prob. 3BYMVCh. 7 - Prob. 4BYMVCh. 7 - Prob. 5BYMVCh. 7 - Prob. 6BYMVCh. 7 - Prob. 7BYMVCh. 7 - Prob. 8BYMVCh. 7 - State a meaning of each word element and give a...Ch. 7 - Prob. 10BYMVCh. 7 - Prob. 1WWWTSCh. 7 - Prob. 2WWWTSCh. 7 - Prob. 3WWWTSCh. 7 - Prob. 4WWWTSCh. 7 - Prob. 5WWWTSCh. 7 - Prob. 6WWWTSCh. 7 - Prob. 7WWWTSCh. 7 - Prob. 8WWWTSCh. 7 - Prob. 9WWWTSCh. 7 - Prob. 10WWWTSCh. 7 - Prob. 1TYCCh. 7 - Prob. 2TYCCh. 7 - Prob. 3TYCCh. 7 - Prob. 4TYCCh. 7 - Prob. 5TYC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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- Why are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forward
- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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