Human Biology: Concepts and Current Issues
Human Biology: Concepts and Current Issues
7th Edition
ISBN: 9780321821652
Author: Michael D. Johnson
Publisher: Benjamin Cummings
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Chapter 7, Problem 5TY
Summary Introduction

Introduction:

WBCs (white blood cells), RBCs (red blood cells), and platelets are the different types of formed elements that remain suspended in the blood plasma. These formed elements, as well as plasma, are essential in carrying out various activities in the body. The deviation in the number of formed elements can occur due to several reasons.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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