Microbiology: An Introduction
Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 7, Problem 4R
Summary Introduction

To review:

Effects of gamma radiation and UV radiation on microorganisms.

Introduction:

Antimicrobial is an agent that kills and stops the growth of microorganisms. Gamma radiation is an ionizing radiation with shorter wave length having the ability to penetrate deeply. UV radiation is a non-ionizing radiation with longer wave length having minimum ability to penetrate.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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