LIFE:SCIENCE OF BIOL.(LL) >CUSTOM<
LIFE:SCIENCE OF BIOL.(LL) >CUSTOM<
11th Edition
ISBN: 9781319209957
Author: Sadava
Publisher: MAC HIGHER
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Chapter 7, Problem 4Q
Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of 32P-EnvZ with OmpR.

Duration after mixing 32P-EnvZ and OmpR (min)
0 2 5 10
Presence of 32P label in EnvZ High Medium Low Absent
Presence of 32P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

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