LIFE:SCIENCE OF BIOL.(LL) >CUSTOM<
LIFE:SCIENCE OF BIOL.(LL) >CUSTOM<
11th Edition
ISBN: 9781319209957
Author: Sadava
Publisher: MAC HIGHER
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Chapter 7, Problem 3Q
Summary Introduction

To review:

Evidence of signal amplification in the signal transduction pathway.

Introduction:

Different genes of a cell get activated in different circumstances. Bacterial cells like Escherichia coli express a high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins, such as membrane protein (EnvZ), act as a protein kinase and get phosphorylated. They also phosphorylate proteins like OmpR (cytoplasm protein).

The amount of EnvZ and OmpR after incubation with 32P-radiolabeled ATP is recorded in the following table:

Table A

An isotonic solution consisting of nutrient broth only A hypertonic solution consisting of Nutrient broth and 20% sucrose solution
EnvZ 63 113
OmpR 2043 3525

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