Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 7, Problem 4Q

Which step can you omit without affecting determination of the Gram reaction?

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Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer. What factor(s) did you take into consideration when selecting the number of passes? Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative? What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a? If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend?
Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to make a 10-1 dilution of the ENTIRE culture. Explain how you would do this.
A mistake was made in preparing the arginine decarboxylase test, and mineral oil was NOT added to the test tube after inoculating with bacteria. If an arginine decarboxylase-positive bacteria species was inoculated into the above arginine-decarboxylase broth without mineral oil, what would likely be the color result of this bacteria in this test? Would this produce a false positive or false negative result? Explain.
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