Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 7, Problem 2CT
Summary Introduction
To conclude:
The condition about viewing gram stained field of red rods and purple cocci with the help of a microscope.
Introduction:
It is a common method that is used to distinguish two large bacterial groups based on their dissimilar cell wall components. The Gram stain process differentiates between the Gram positive and negative bacterial groups by coloring these cells violet or red. The dyes used in gram staining are useful for staining of the peptidoglycan in the bacterial cell wall.
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Chapter 7 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 7 - Why do gram-positive cells more than 24 hours old...Ch. 7 - Can iodine be added before the primary stain in a...Ch. 7 - Prob. 3QCh. 7 - Which step can you omit without affecting...Ch. 7 - Suppose you performed a Gram stain on a sample...Ch. 7 - Prob. 2CTCh. 7 - Human cells can be stained with crystal violet and...Ch. 7 - Considering that it isnt possible to identity...
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- Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. At 100X you are unable to see the diatom. Explain why. Explain what correction (s) should be made to allow you to see the diatom using the 100X lens. After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred.arrow_forwardAn acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following couid explain why only blue cells were present?arrow_forwardUsing an appropriate lens and oil immersion, what would be a microscope's maximum resolution (in micrometres) that could use a wavelength of 350 nm (assuming that such a device exists)? What would be the surface-to-volume ratio for the coccus-shaped bacterium twice the size of the maximum resolution you calculated? Why is the surface-to-volume ratio an important biological factor. Explain. Show all of your calculations.arrow_forward
- a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?arrow_forwardWhy is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.arrow_forwardSuppose you are staining three identical smears of the same bacterial culture with three different basic simple stains: carbol fuschin, crystal violet and methylene blue. You observe each stained preparation under oil immersion at 100X. which of the following bacterial characteristics would you expect to be different between each of the stains as you view them under the microscope. Bacterial arrangement Bacterial shape Size of cells The color of the bacteriaarrow_forward
- if a bacteria under observation in a microscope appears to be blurry, what measures are needed to do in the microscope in order to see and identify it clearly?arrow_forwardJanet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.arrow_forwardExamine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information A. Shape ........; Gram stain ( Gram (+), Gram (-) or both ... B. Stained color under microscope..... . C. Is the PG wall think, thin or both ..arrow_forward
- The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forwardDescribe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)arrow_forwardIf you were working with an unlabeled simple stained smear, would you be able to identify the bacterial species by observing the slide under the microscope? Why or why not?arrow_forward
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