Considering the results of DNA replication analysis of living microbe from Mars conclude about the mechanism of DNA replication in this organism. Introduction: Meselson and Stahl performed an experiment to identify the mechanism of DNA replication in any known or unknown organism. In this experiment, an organism that possessed double stranded DNA was allowed to grow on a medium containing 15 N (Heavy isotope of nitrogen); after that, they transferred the organism to a medium containing 14 N (Light isotope of nitrogen). When DNA is isolated from the experimental organism after 1 replication cycle and C s Cl ultracentrifugation followed by densitometry is performed, it shows intermediate density ( 15 N 14 N ) DNA. From this result, Meselson and Stahl concluded that the process of DNA replication is semi-conservative.

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Chapter 7, Problem 35P

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Considering the results of DNA replication analysis of living microbe from Mars conclude about the mechanism of DNA replication in this organism.

Introduction:

Meselson and Stahl performed an experiment to identify the mechanism of DNA replication in any known or unknown organism. In this experiment, an organism that possessed double stranded DNA was allowed to grow on a medium containing 15N (Heavy isotope of nitrogen); after that, they transferred the organism to a medium containing 14N (Light isotope of nitrogen). When DNA is isolated from the experimental organism after 1 replication cycle and CsCl ultracentrifugation followed by densitometry is performed, it shows intermediate density (15N 14N) DNA. From this result, Meselson and Stahl concluded that the process of DNA replication is semi-conservative.

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