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To analyze:
Considering the results of
Introduction:
Meselson and Stahl performed an experiment to identify the mechanism of DNA replication in any known or unknown organism. In this experiment, an organism that possessed double stranded DNA was allowed to grow on a medium containing
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Chapter 7 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
- please answer the following questionarrow_forwardSuppose you have a cell-lysis sample containing genomic DNA and a complex mixture of proteins and debris. Describe different commonly used analytical methods to i) separate and purify DNA contents from other cellular contents. ii) amplify a gene of interest if present in the genomic DNA.arrow_forwardassume standard volumes, it is complete question.arrow_forward
- The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.arrow_forwardEngineered bacteria are commonly used and cultured in pharmaceutical laboratories to produced drugs like insulin. However, during one instance, with the desire to increase the rate of insulin production, one scientist tried to modify the process and put another substance (chemical x) in the growth medium which is chemically almost the same to the substance (chemical x’) normally put in the medium. After which, the scientist found out that the amount of insulin produced in the medium decreases as compared to the normal production. Again, within the context of our discussion, explain the possible reason for the observed result.arrow_forward"the supplement that restored growth would be the molecule that the mutant strain could not synthesize" Explain this statement ?arrow_forward
- Bacterial endospores form when the environment is no longer conducive to active cell metabolism. The soil bacterium Bacillus subtilis, for example, begins the process of sporulation when one or more nutrients aredepleted. The end product is a small, metabolically dormant structure that can survive almost indefinitely with no detectable metabolism. Spores have mechanisms to prevent accumulation of potentially lethal mutations in their DNA over periods of dormancy that can exceed 1,000 years. B. subtilis spores are much more resistant than are the organism’s growing cells to heat, UV radiation, and oxidizing agents, all of which promote mutations.(a) One factor that prevents potential DNA damage in spores is their greatly decreased water content. How would this affect some types of mutations?(b) Endospores have a category of proteins called small acid-soluble proteins (SASPs) that bind to their DNA, preventing formation of cyclobutane-type dimers. What causes cyclobutane dimers, and why…arrow_forwardExplain Avery-MacLeod-McCarty experiment (the 1940's) with capsule forming bacteria (smooth) and non-capsule forming bacteria (rough). What did this classic experiment demonstrate?Why would a rapidly growing bacterial cell be killed by an antibiotic that blocks DNA replication? Suppose the bacteria form a biofilm on a catheter in a patient. Why might the bacteria have become resistant to the antibiotic?arrow_forwardIt is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?arrow_forward
- please do (ii)arrow_forwardSome bacteria may have higher mutation rates than others following exposure to UV light. Discuss a reason why this might be the case. What experiments could you do to determine whether this is a likely possibility?arrow_forwardThe synthesis of arginine by Nuerospora was determined by examining a number of mutant strains that were unable to synthesize the compound. Use the table of bacterial growth below to 1) determine the correct sequence of the synthesis pathway and 2) where in the synthesis pathway each mutation interrupts the synthesis. A “+" indicates growth. Nothing added to Succinate Ornithine added Strain Cirtulline Arginine Added added added growth medium Wild Mutant 1 Mutant 2 Mutant 3 Mutant 4arrow_forward
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