GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Textbook Question
Chapter 7, Problem 14P
Bacterial DNA polymerase I and DNA polymerase III per-form different functions during
a. Identify the principal functions of each molecule.
b. If mutation inactivated DNA polymerase I in a strain of E. coli, would the cell be able to replicate its DNA? If so, what kind of abnormalities would you expect to find in the cell?
c. If a strain of E. coli acquired a mutation that inactivated DNA polymerase III function, would the cell be able to replicate its DNA? Why or why not?
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Students have asked these similar questions
a. As a result of the structure of DNA and RNA, replication, transcription and translation are possible. What can nucleic acids do, as a result of their structure, that enables
these processes to occur?
The figure below shows a simplified schematic representation of a segment of DNA. The DNA is labelled with the numbers 1 – 14 for easy reference.
-35 sequence
Pribnow box
5' UTR
3' UTR
DNA
TTGACA
TATAAT
-35
-10
Gene a
Gene B
Gene y
1
2
3
4
5
6
7
8 9
10 11
12
13
14
UTR = untranslated region
b. At which position on the DNA (number 1 - 14) will transcription be initiated?
c. At which position on the DNA (number 1 - 14) will the first signal for translation be found?
d. Between which two regions on the DNA will the polyadenylation signal be found? Use the numbers to indicate the region.
e. Between which two regions on the DNA will the first Shine-Dalgarno / Ribosome Binding Sequence be found? Use the numbers to indicate the region.
a. What process would be unaffected in with defective topoisomerase?
Prokaryotic DNA packaging
Eukaryotic DNA packaging
Prokaryotic DNA replication
Eukaryotic DNA replication
All of the above rely on topoisomerase function
b. Why is primase required for DNA replication?
To unwind the DNA helix
To prime nucleotides for addition to the growing chain
To provide a 3' OH for DNA polymerase
To recognize the origin of replication
To remove supercoils ahead of the replication machinery
C. What is the purpose of PCR reaction?
To alter the sequence of a fragment of DNA.
To insert a fragment of DNA into a bacterium
To amplify a fragment of ONA
To destroy a fragment of DNA
To determine the sequence of nucleotides in a fragment of DNA.
Please consider figures 1 and 2 below.
5'
A
B
5' -I
H.
D.
Figure 1: Replication
Figure 2: Transcription
a. Which letters, A to J represent DNA strands that will be synthesized continuously?
b. At which letter, A to J, are ribonucleotides being added?
c. Name and describe the process that occurs in prokaryotes as the synthesized mRNA hangs free of the double helix while transcription continues.
d. In what direction will the replication bubble move in figure 1 as replication continues?
Rleace Ibol
ancwors (a) to (d)
Chapter 7 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
Ch. 7 - What results from the experiments of Frederick...Ch. 7 - 7.2 Explain why Avery, MacLeod, and McCarty’s in...Ch. 7 - 7.3 Hershey and Chase selected the bacteriophage...Ch. 7 - 7.4 Explain how the Hershey and Chase experiment...Ch. 7 - 7.5 One strand of a fragment of duplex DNA has the...Ch. 7 - 7.6 The principles of complementary base pairing...Ch. 7 - For the following fragment of DNA, determine the...Ch. 7 - 7.8 Figures present simplified depictions of...Ch. 7 - 7.9 Consider the sequence -ACGCTACGTC-.
What is...Ch. 7 - DNA polymerase III is the main DNA-synthesizing...
Ch. 7 - Explain how RNA participates in DNA replication.Ch. 7 - A sample of double-stranded DNA is found to...Ch. 7 - Bacterial DNA polymerase I and DNA polymerase III...Ch. 7 - Diagram a replication fork in bacterial DNA and...Ch. 7 - Prob. 16PCh. 7 - Which of the following equalities is not true for...Ch. 7 - List the order in which the following proteins and...Ch. 7 - Two viral genomes are sequenced, and the following...Ch. 7 - Matthew Meselson and Franklin Stahl demonstrated...Ch. 7 - Raymond Rodriguez and colleagues demonstrated...Ch. 7 - 7.22 Joel Huberman and Arthur Riggs used pulse...Ch. 7 - 7.23 Why do the genomes of eukaryotes, such as...Ch. 7 - Bloom syndrome (OMIM 210900) is an autosomal...Ch. 7 - 7.25 How does rolling circle replication (see...Ch. 7 - Telomeres are found at the ends of eukaryotic...Ch. 7 - A family consisting of a mother (I-1), a father...Ch. 7 - In a dideoxy DNA sequencing experiment, four...Ch. 7 - Prob. 29PCh. 7 - Using an illustration style and labeling similar...Ch. 7 - A PCR reaction begins with one double-stranded...Ch. 7 - Prob. 32PCh. 7 - Three independently assorting VNTR markers are...Ch. 7 - 7.34 A sufficient amount of a small DNA fragment...Ch. 7 - Prob. 35P
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- (b): How many forms can naturally occurring DNA exist in? Explain how these forms are characterized. What properties of the functional groups determine the binding of DNA with anti-cancer agents? Explain.arrow_forwardQ.Sheltrin protein complex that has the following function: a.Protect telomerase from damage b.Protect telomere from damage c.Proteins as chaperones in autophagy d.Responsible in Proteostasis Q.Telomeres shorten by 50-200 bases with every cell division due to. a.End replication problem b.Cellular senescence c.mutations of Sheltrin proteins d.DNA Recombinationarrow_forwardI. Compare how Bacteria, Archaea, and Eukaryotes differ on each of the following aspects of DNA replication: 1. How do the number and types of DNA polymerase differ between these three groups? 2. How does the physical location in the cell of DNA replication differ between these three groups? 3. Are there differences between these three groups in the timing of when DNA replication occurs in cells? 4. How does origin of replication differ in terms of number and location between these three groups?arrow_forward
- A.) There is no change in the DNA sequence if nucleotides are added or removed, it will have no effect to the cell. B.) Mutations in the DNA sequence are all irreversible. A. Statement A is correct B. Statement B is correct C. Both A and B are correct D. Both A and B are incorrectarrow_forward5) In DNA replication, which of the following events happens during both leading and lagging strand synthesis? a. RNA primers help initiate DNA synthesis. b. Topoisomerase separates complementary strands of DNA. c. Ligase joins together multiple Okazaki fragments. d. DNA polymerase synthesizes a single, continuous strand of DNA. Explain WHY? 6) Suppose you are provided with an actively dividing culture of E. coli bacteria to which radioactive thymine has been added. What would happen if a cell replicates once in the presence of this radioactive base? a. One of the daughter cells, but not the other, would have radioactive DNA. b. Neither of the two daughter cells would be radioactive. c. All four bases of the DNA would be radioactive. d. DNA in both daughter cells would be radioactive. Explain WHY?arrow_forward1 2 3arrow_forward
- a. What DNA sequences are found at the telomeresof human chromosomes?b. What functions do the two telomere-associatedcomplexes, telomerase and shelterin, fulfill at chromosome ends?c. Where do you think that the RNA component oftelomerase comes from?arrow_forwarda) Under normal conditions E. coli produces three DNA polymerases. State their functional similarities and differences. b) List the other proteins and enzymes involved in DNA replication in E.coli and give their functions.arrow_forwardDescribe three factors that account for the high fidelity of DNA replication.Discuss the quantitative contributions of each of the three.arrow_forward
- explain the laws of DNA polymerase? Why are they important and are all the laws required for DNA polymerase to function?arrow_forwarda) Explain semi-conservative modelmof DNA replication b) discuss initiation in DNA replication and explain why it is a tightly controlled process.arrow_forward, describe the normal role of the component, and choose from the selection the most direct effect of the mutation and explain WHY. Choose from the 4 following effects: Initiation of DNA synthesis okazaki fragment synthesis Leading strand elongation lagging-strand completion --> Explain why these mutant strains are most likely temperature-sensitive mutantsarrow_forward
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