Concept explainers
Videos
(a)
To explain: The reason behind no antigen-antibody precipitate forms when Fab fragments from antibodies are mixed with the antigen.
Concept introduction: Antibodies are produced by the plasma cells and also known as immunoglobulins that help to remove the invading pathogen. The antibodies are also used for various purposes that include identifying or locating protein of interest and many more. The native proteins are antigens that are already present in the cell. The antigen is any molecule that triggers the immune system to elicit an immune response.
(b)
To explain: The reason behind no antigen-antibody precipitate forms when antibodies raised against a small antigen are mixed with that small antigen.
Concept introduction: Antibodies are produced by the plasma cells and also known as immunoglobulins that help to remove the invading pathogen. The antibodies are also used for various purposes that include identifying or locating protein of interest and many more. The native proteins are antigens that are already present in the cell. The antigen is any molecule that triggers the immune system to elicit an immune response.
(c)
To explain: The reason behind no antigen-antibody precipitate forms when the antibody is in great excess over the antigen and vice versa.
Concept introduction: Antibodies are produced by the plasma cells and also known as immunoglobulins that help to remove the invading pathogen. The antibodies are also used for various purposes that include identifying or locating protein of interest and many more. The native proteins are antigens that are already present in the cell. The antigen is any molecule that triggers the immune system to elicit an immune response.
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Chapter 7 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
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- We always include controls in the Annexin-V-GFP/Propidium Iodide flow cytometric assay to study apoptosis. List four types of controls in this assay. Why do we need these controls? Explain your answers. After the flow assay, if we like to examine the morphology of the viable, early apoptotic and late apoptotic cells by confocal microscopy, what can we do and what are the expected results?arrow_forward3. (2 points) Your lab partner accidentally used a pen instead of a pencil to mark the baseline and label the lanes of their TLC plate. Briefly (1-2 sentences for each point) describe (a) what would happen to the ink when you develop the TLC plate; and (b) how this would affect the experiment. 1arrow_forwardCan you help me with question 4arrow_forward
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