EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
7th Edition
ISBN: 8220103662253
Author: nelson
Publisher: MAC HIGHER
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Chapter 7, Problem 25P
Summary Introduction

To draw: The gel pattern of a mixture of four glycoprotein’s containing zero, one, two or three oligosaccharide chains in SDS PAGE and also identifies the bands.

Introduction: Oligosaccharide is a polymer of saccharide units. Oligosaccharide molecules are made up of three to ten monosaccharide units. These monosaccharide units are joined to each other through glycosidic linkages.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.  The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY