Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 6.3, Problem 2LO
Summary Introduction
To distinguish: The bonds in ATP that have “high energy”.
Introduction: Adenosine triphosphate (ATP) is an organic compound that gives energy to drive several processes in the living cells. For example, contraction of muscle, propagation of nerve impulses, and chemical synthesis. The ATP is often referred to as the energy currency for the cell. This is because the energy released by ATP hydrolysis can control most of the endergonic transactions.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 6 Solutions
Biology
Ch. 6.1 - Prob. 1LOCh. 6.1 - Prob. 2LOCh. 6.1 - Describe the nature of redox reactions.Ch. 6.2 - Explain the laws of thermodynamics.Ch. 6.2 - Prob. 2LOCh. 6.2 - Contrast the course of a reaction with and without...Ch. 6.3 - Describe the role of ATP in short-term energy...Ch. 6.3 - Prob. 2LOCh. 6.4 - Discuss the specificity of enzymes.Ch. 6.4 - Explain how enzymes bind to their substrates.
Ch. 6.4 - Prob. 3LOCh. 6.5 - Prob. 1LOCh. 6.5 - Prob. 2LOCh. 6.5 - Prob. 3LOCh. 6 - Prob. 1DACh. 6 - A covalent bond between two atoms represents what...Ch. 6 - During a redox reaction the molecule that gains an...Ch. 6 - Prob. 3UCh. 6 - A spontaneous reaction is one in which a. the...Ch. 6 - Prob. 5UCh. 6 - Which of the following is NOT a properly of a...Ch. 6 - Where is the energy stored in a molecule of ATP?...Ch. 6 - Prob. 1ACh. 6 - Which of the following statements is NOT true...Ch. 6 - Prob. 3ACh. 6 - Prob. 4ACh. 6 - Enzymes have similar responses to both changes in...Ch. 6 - Prob. 6ACh. 6 - Examine the graph showing the rate of reaction...Ch. 6 - Phosphofructokinase functions to add a phosphate...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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