The reason for solvent to not allow evaporating from the plate during development should be explained. Concept introduction: Thin Layer Chromatography is a sensitive chromatographic technique used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent; sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

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Chapter 6.2, Problem 4E

Interpretation Introduction

Interpretation: The reason for solvent to not allow evaporating from the plate during development should be explained.

Concept introduction: Thin Layer Chromatography is a sensitive chromatographic technique used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent; sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

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