Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 6.1, Problem 1NP
Summary Introduction
Introduction:
The mode of nutrition is an important classification among bacteria. On the basis of the source of energy and carbon source the organisms are classified into two major categories autotrophs and heterotrophs. Autotrophs use the sunlight, organic and inorganic sources as energy sources and CO2 as the carbon source, heterotrophs on the other hand use organic matter as the source of energy as well as the carbon source.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 6 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 6.1 - List the essential nutrients of a bacterial cell.Ch. 6.1 - Prob. 2AYPCh. 6.1 - List and define four different terms that describe...Ch. 6.1 - Prob. 4AYPCh. 6.1 - Compare and contrast the processes of diffusion...Ch. 6.1 - Identify the effects of isotonic, hypotonic, and...Ch. 6.1 - Name two types of passive transport and one type...Ch. 6.1 - Prob. 1NPCh. 6.1 - Prob. 2NPCh. 6.1 - Prob. 3NP
Ch. 6.1 - Prob. 1MMCh. 6.2 - List and define five terms used to express the...Ch. 6.2 - Summarize three ways in which microorganisms...Ch. 6.2 - Identify three important environmental factors...Ch. 6.2 - List and describe the five types of associations...Ch. 6.2 - Discuss characteristics of biofilms that...Ch. 6.2 - Which statements are true with respect to...Ch. 6.3 - Summarize the steps of cell division used by most...Ch. 6.3 - Define doubling time, and describe how it leads to...Ch. 6.3 - Compare and contrast the four phases of growth in...Ch. 6.3 - Identify one culture-based and one...Ch. 6.3 - Prob. 2MMCh. 6 - Which descriptors are likely to have applied to...Ch. 6 - Prob. 2QCh. 6 - Speculate about how earths atmosphere came to...Ch. 6 - Which of the following is true of passive...Ch. 6 - Compare the effects of a hypertonic, hypotonic,...Ch. 6 - Usually scientists looking for life on other...Ch. 6 - An organism that can synthesize all its required...Ch. 6 - Provide evidence in support of or refuting this...Ch. 6 - Develop an explanation for why biofilm bacteria...Ch. 6 - Most bacteria increase their numbers by a. sexual...Ch. 6 - Looking at figure 6.3, explain how a cell in a...Ch. 6 - In binary fission, the parent chromosome is...Ch. 6 - A cell exposed to a hypertonic environment will...Ch. 6 - Prob. 14QCh. 6 - Bacteria and archaea are ubiquitous on the planet....Ch. 6 - Prob. 16QCh. 6 - How can you explain the fact that an unopened...Ch. 6 - Prob. 18QCh. 6 - In a viable count, each ____ represents a ______...Ch. 6 - If an egg salad sandwich sitting in a car on a...Ch. 6 - Scientists now believe that most bacteria in...Ch. 6 - Prob. 1VC
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