Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 6, Problem 9R
DRAW IT Draw the following growth curves for E. coli, starting with 100 cells with a generation time of 30 minutes at 35°C, 60 minutes at 20°C, and 3 hours at 5°C.
- a. The cells are incubated for 5 hours at 35°C.
- b. After 5 hours, the temperature is changed to 20°C for 2 hours.
- c. After 5 hours at 35°C, the temperature is changed to 5°C for 2 hours followed by 35°C for 5 hours.
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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
E.coli was incubated with aeration in a nutrient medium containing two carbon sources provided one at a time, and the following growth curve was made from this culture.
a. Explain what happened at the time marked x.
b. Which substrate provided “better” growth conditions for the bacteria? How can you tell?
Chapter 6 Solutions
Microbiology: An Introduction
Ch. 6 - Prob. 1RCh. 6 - Prob. 2RCh. 6 - Prob. 3RCh. 6 - Seven methods of measuring microbial growth were...Ch. 6 - By deep-freezing, bacteria can be stored without...Ch. 6 - A pastry chef accidentally inoculated a cream pie...Ch. 6 - Nitrogen and phosphorus added to beaches following...Ch. 6 - Differentiate complex and chemically defined...Ch. 6 - DRAW IT Draw the following growth curves for E....Ch. 6 - NAME IT A prokaryotic cell hitched a ride to Earth...
Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Assume you inoculated 100 facultatively anaerobic...Ch. 6 - The term trace elements refers to a. the elements...Ch. 6 - Which one of the following temperatures would most...Ch. 6 - Which of the following is not a characteristic of...Ch. 6 - Which of the following types of media would not be...Ch. 6 - An organism that has peroxidase and superoxide...Ch. 6 - E. coli was incubated with aeration in a nutrient...Ch. 6 - Clostridium and Streptococcus are both...Ch. 6 - Most laboratory media contain a fermentable...Ch. 6 - Flask A contains yeast cells in glucose-minimal...Ch. 6 - Clinical Applications and Evaluation 1. Assume...Ch. 6 - Heat lamps are commonly used to maintain foods at...Ch. 6 - The number of bacteria in saliva samples was...
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