Microbiology: An Introduction
Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 6, Problem 3CAE

The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar by the pour plate method. The plates were incubated aerobically for 48 hours at 37°C.

  Bacteria per ml Saliva
  Before UsingMouthwash After UsingMouthwash
Mouthwash 1 13.1 × 106 10.9 × 106
Mouthwash 2 11.7 × 106 14.2 × 105
Mouthwash 3 9.3 × 105 7.7 × 105

What can you conclude from these data? Did all the bacteria present in each saliva sample grow?

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13. Practice Calculations: 3 colonies were suspended in the following dilution series and then a viable plate count and microscope count was performed. Calculate IDF's, TDF's and then calculate the CFU/mL in each tube by both methods. Finally calculate the cells in 1 colony by both methods. Show all of your calculations in the space provided on the following pages. 3 colonies 56 cells 10 μL 10 μL 100 μL 500 με m OS A B D 5.0 mL 990 με 990 με 900 με 500 μL EN 2 100 με 100 μL 118 colonies 12 colonies

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Microbiology: An Introduction

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