
Concept explainers
A.
To explain: What would happen if dideoxycytosine triphosphate (ddCTP) were added in excess over the concentration of available normal deoxycytosine triphosphate (dCTP) in a
Introduction: DNA is the blueprint of life and contains genetic information. The replication of DNA is semiconservative. During DNA replication, DNA polymerase enzyme molecules catalyze the synthesis of DNA. They add DNA nucleotides to an existing strand at 3′-OH in the 5ʹ-3ʹ direction with high fidelity and proofread it. The nucleotides enter the DNA replication reaction as deoxyribonucleoside triphosphate.
B.
To explain: What would happen if ddCTP were added at 10% of the concentration of the available dCTP.
Introduction: DNA is the blueprint of life and contains genetic information. The replication of DNA is semiconservative. During DNA replication, DNA polymerase enzyme molecules catalyze the synthesis of DNA. They add DNA nucleotides to an existing strand at 3′-OH in the 5ʹ-3ʹ direction with high fidelity and proofread it. The nucleotides enter the DNA replication reaction as deoxyribonucleoside triphosphate.
C.
To explain: What would happen if dideoxycytosine monophosphate (ddCMP) were added to a DNA replication reaction?
Introduction: DNA is the blueprint of life and contains genetic information. The replication of DNA is semiconservative. During DNA replication, DNA polymerase enzyme molecules catalyze the synthesis of DNA. They add DNA nucleotides to an existing strand at 3′-OH in the 5ʹ-3ʹ direction with high fidelity and proofread it. The nucleotides enter the DNA replication reaction as deoxyribonucleoside triphosphate.

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