IA MODIFIED MASTERING BIOLOGY WITH E TEX
12th Edition
ISBN: 9780136781752
Author: Urry
Publisher: PEARSON
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Chapter 6, Problem 8TYU
SCIENTIFIC INQUIRY Imagine protein X, destined to span the plasma membrane. Assume that the mRNA carrying the genetic message for protein X has already been translated by ribosomes in a cell culture. If you fractionate the cells (see Figure 6.4), in which fraction would you find protein X? Explain by describing its transit through the cell.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 6 Solutions
IA MODIFIED MASTERING BIOLOGY WITH E TEX
Ch. 6.1 - Prob. 1CCCh. 6.1 - Prob. 2CCCh. 6.2 - Briefly describe the structure and function of the...Ch. 6.2 - DRAW IT Draw a simplified elongated cell that...Ch. 6.3 - What role do ribosomes play in carrying out...Ch. 6.3 - Describe the molecular composition of nucleoli and...Ch. 6.3 - Prob. 3CCCh. 6.4 - Describe the structural and functional...Ch. 6.4 - Describe how transport vesicles integrate the...Ch. 6.4 - WHAT IF? Imagine a protein that functions in the...
Ch. 6.5 - Describe two characteristics shared by...Ch. 6.5 - Prob. 2CCCh. 6.5 - Prob. 3CCCh. 6.6 - Prob. 1CCCh. 6.6 - WHAT IF? Males afflicted with Kartagener's...Ch. 6.7 - In what way are the cells of plants and animals...Ch. 6.7 - Prob. 2CCCh. 6.7 - MAKE CONNECTIONS The polypeptide chain that makes...Ch. 6.8 - Prob. 1CCCh. 6 - Prob. 6.1CRCh. 6 - Explain how the compartmental organization of a...Ch. 6 - Describe the relationship between the nucleus and...Ch. 6 - Describe the key role played by transport vesicles...Ch. 6 - What does the endosymbiont theory propose us the...Ch. 6 - Describe the role of motor proteins inside the...Ch. 6 - Prob. 6.7CRCh. 6 - When a cell ingests a bacterium, what role does...Ch. 6 - Prob. 1TYUCh. 6 - Prob. 2TYUCh. 6 - Which of the following is present in a prokaryotic...Ch. 6 - Prob. 4TYUCh. 6 - Which cell would be best for studying lysosomes?...Ch. 6 - Prob. 6TYUCh. 6 - EVOLUTION CONNECTION (a) What cell structures best...Ch. 6 - SCIENTIFIC INQUIRY Imagine protein X, destined to...Ch. 6 - WRITE ABOUT A THEME: ORGANIZATION Considering some...Ch. 6 - SYNTHESIZE YOUR KNOWLEDGE The cells in this SEM...
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