To draw: The two eukaryotic cells. Introduction: Plants and animals are both eukaryotes. They have well-organized membrane-bound structures. A cell contains different organelles. Each organelle has its own specific features. Plant cell walls are the outermost boundary of plant cells that provide shape and protection to the cell. Animal cells do not have cell walls but consist of a plasma membrane. The cell synthesizes and secretes materials outside of cells, called extracellular matrix (ECM) that plays a role in cellular functions and protection.

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 6, Problem 8TYU

Summary Introduction

To draw: The two eukaryotic cells.

Introduction: Plants and animals are both eukaryotes. They have well-organized membrane-bound structures. A cell contains different organelles. Each organelle has its own specific features. Plant cell walls are the outermost boundary of plant cells that provide shape and protection to the cell. Animal cells do not have cell walls but consist of a plasma membrane. The cell synthesizes and secretes materials outside of cells, called extracellular matrix (ECM) that plays a role in cellular functions and protection.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 6, Problem 8TYU

Summary Introduction

To draw: The two eukaryotic cells.

Introduction: Plants and animals are both eukaryotes. They have well-organized membrane-bound structures. A cell contains different organelles. Each organelle has its own specific features. Plant cell walls are the outermost boundary of plant cells that provide shape and protection to the cell. Animal cells do not have cell walls but consist of a plasma membrane. The cell synthesizes and secretes materials outside of cells, called extracellular matrix (ECM) that plays a role in cellular functions and protection.

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Answer 3

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 6, Problem 8TYU

Summary Introduction

To draw: The two eukaryotic cells.

Introduction: Plants and animals are both eukaryotes. They have well-organized membrane-bound structures. A cell contains different organelles. Each organelle has its own specific features. Plant cell walls are the outermost boundary of plant cells that provide shape and protection to the cell. Animal cells do not have cell walls but consist of a plasma membrane. The cell synthesizes and secretes materials outside of cells, called extracellular matrix (ECM) that plays a role in cellular functions and protection.

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Answer 4

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 6, Problem 8TYU

Summary Introduction