LAUNCHPAD F/WHAT IS LIFE
4th Edition
ISBN: 9781319231897
Author: PHELAN
Publisher: Macmillan Higher Education
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Chapter 6, Problem 8SA
Summary Introduction
To analyze:
How E. coli is able to survive in an environment with lactose and no glucose.
Introduction:
Although E. coli uses glucose as its primary source of energy, it has alternate pathways by which it can metabolize lactose.
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When lactose is added to growing culture of E. coli, the cells begin to make enzymes necessary for lactose utilization. In contrast, when tryptophan is added to the culture medium, synthesis of the enzymes for tryptophan pathway ceases. Contrast the two systems explaining why they act in reverse directions
The beaches at a nearby lake have been closed because of high E. coli counts. The lake is surrounded by agricultural areas and summer cottages. We know that different species of vertebrates have different E. coli strains in their digestive tracts. Propose a hypothesis about the source of the E. coli and design an experiment to test this hypothesis.
How many of these strains of E. coli would have no ß-galactosidase activity?
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