Chapter 6, Problem 3CT

Summary Introduction

To write:

Acidic congo red does not have the suspended particles unlike the nigrosin butprovides the presence of a negative stain. Give the basis for this stain.

Introduction:

Heat fixing:

After the drying of smear at the room temperature on the slide. This slide is gripped with the help of tongs or a clothespin. Then it is passed across the flame of a Bunsen burner and numerous times to heat-kill. It attaches the organism to the slide. Heat fixation usually conserves generally the morphology but not an internal structure.