Microbiology with Diseases by Body System (4th Edition)
4th Edition
ISBN: 9780321918550
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 6, Problem 21CT
Summary Introduction
To categorize:
The two media given in Tables 6.3 and 6.4 using the terms—selective, defined, differential, broth, complex, and solid.
Introduction:
A growth medium is used for culturing microorganisms. The media can be solid, liquid, or semi-solid (in some cases). A variety of media are available for culturing various types of cells. The eosin methylene blue (EMB) agar is an example of selective differential complex solid medium, and triple sugar iron (TSI) agar is a differential complex solid media.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks)
a) How would you prepare a 500 ml nutrient broth?
b) How would you prepare 2% (w / v) nutrient agar for 1L?
c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .
Microbial products can be classified as three major categories. With the use of sketch, briefly explain the kinetic patterns of growth and product formation in batch fermentation.
Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.
Chapter 6 Solutions
Microbiology with Diseases by Body System (4th Edition)
Ch. 6 - Why should cardiac nurses and respiratory...Ch. 6 - Cavities Gone Wild Five-year-old Daniel appears to...Ch. 6 - Why do clinical laboratory scientists keep many...Ch. 6 - Boils in the Locker Room For several weeks,...Ch. 6 - Some students transfer some gunk from a 2-week-old...Ch. 6 - Which of the following can grow in a Petri plate...Ch. 6 - In the laboratory, a sterile inoculating loop is...Ch. 6 - Superoxide dismutase _____________. a. causes...Ch. 6 - The most reactive of the four toxic forms of...Ch. 6 - Microaerophiles that grow best with a high...
Ch. 6 - Prob. 6MCCh. 6 - Organisms that preferentially may thrive in icy...Ch. 6 - Prob. 8MCCh. 6 - Which of the following terms best describes an...Ch. 6 - In a defined medium, ______________. a. the exact...Ch. 6 - Which of the following is most useful in...Ch. 6 - Which of the following methods is best for...Ch. 6 - A Coulter counter is a(n) ________________. a....Ch. 6 - Lyophilization can be described as ___________. a....Ch. 6 - Quorum sensing is _____________. a. the ability to...Ch. 6 - Prob. 1FIBCh. 6 - Prob. 2FIBCh. 6 - Prob. 3FIBCh. 6 - Prob. 4FIBCh. 6 - Prob. 5FIBCh. 6 - Fill in the Blanks 6. Cells that shrink in...Ch. 6 - Fill in the Blanks 7. Obligate ________ exist in...Ch. 6 - Prob. 8FIBCh. 6 - Fill in the Blanks 9. Microbes that reduce N2 to...Ch. 6 - Fill in the Blanks 10. A student observes a...Ch. 6 - Fill in the Blanks 11. Chemolithotrophs acquire...Ch. 6 - Prob. 1VICh. 6 - Prob. 2VICh. 6 - High temperature affects the shape of particular...Ch. 6 - Support or refute the following statement:...Ch. 6 - Explain quorum sensing, and describe how it is...Ch. 6 - Why must media, vessels, and instruments be...Ch. 6 - Why is agar used in microbiology?Ch. 6 - What is the difference between complex media and...Ch. 6 - Draw and label the four distinct phases of a...Ch. 6 - If there are 47 cells in 1 l of sewage, how many...Ch. 6 - Prob. 9SACh. 6 - Prob. 10SACh. 6 - Explain the differences among photoautotrophs,...Ch. 6 - Contrast the media described in Tables 6.3 and 6.4...Ch. 6 - How does a chemostat maintain a constant...Ch. 6 - A scientist describes an organism as a...Ch. 6 - Pasteurization is a technique that uses...Ch. 6 - Two cultures of a facultative anaerobe are grown...Ch. 6 - Some organisms require riboflavin (vitamin B2) to...Ch. 6 - A scientist inoculates a bacterium into a complex...Ch. 6 - How can regions within biofilms differ in their...Ch. 6 - A scientific article describes a bacterium as an...Ch. 6 - Prob. 8CTCh. 6 - Prob. 9CTCh. 6 - Starting with 10 bacterial cells per milliliter in...Ch. 6 - Suppose you perform a serial dilution of 0.1-ml...Ch. 6 - How might the study of biofilms benefit humans?Ch. 6 - The filamentous bacterium Beggiatoa gets its...Ch. 6 - Given that Haemophilus ducreyi is a...Ch. 6 - Examine the graph in Figure 6.3. Note that the...Ch. 6 - Prob. 16CTCh. 6 - Using the terms in Figure 6.8a, describe the...Ch. 6 - Prob. 18CTCh. 6 - Prob. 19CTCh. 6 - Prob. 20CTCh. 6 - Prob. 21CTCh. 6 - Prob. 22CTCh. 6 - Viable plate counts are used to estimate...Ch. 6 - Prob. 1CM
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Define three physical states of media and explain when each is useful.arrow_forwardName at least one reason that we might want to use a Winogradsky column rather than a laboratory medium such as nutrient agar or beef heart agar. Describe why metabolism in the presence of oxygen might cause problems for microorganisms.arrow_forward'You have been working in the Quality Control lab at KOO [] for a month when you noticed a dramatic increase in the incidence of thermophilic incubator spoilage of canned sweet corn produced by the company. Describe the steps you would use to determine the cause of the increased spoilage. Your discussion must include the procedure(s) you would use to determine the thermal death time and the D-value of the spoilage organism. NB: Include your own diagrams as well. :arrow_forward
- Nicotiana tabacum cells are cultured to produce a polysaccharide gum. The reactor used is a stirred-tank reactor with an initial volume of 100 L. The maximum specific rate of growth of the culture is 0.18 d-1 and the yield coefficient of substrate in biomass is 0.5 gX/gS. The concentration of the limiting substrate in the medium is 3% (m/v). The reactor is inoculated with 1.5 g/L of cells and operated in batch until the substrate is exhausted, when the medium is fed with a constant flow rate of 8 L/d. The fed batch occurs in a quasi-steady state condition.a) Estimate the time of the batch step and the concentration of cells reached in this phase, considering exponential cell growth.b) The fed batch phase is carried out for a period of 40 days. What is the final concentration of cells in the reactor?c) The bioreactor is available for the process for 275 days a year, with an interval of 24 hours between each cultivation. What is the most advantageous operating mode for the process…arrow_forwardhow are microorganisms are involved in the production of lagers? include the process of bioconversion, biomass or metabolite production, and the specific microorganism or cell structure involved.arrow_forwardNutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non- fastidious microorganisms (Merck, 2000). Its ingredients are listed in Table 2. You are tasked to prepare 300ml of NA, determine the amount of ingredients and write in column 3 in Table 2. Show your calculations. *A sample calculation was made for you. Peptone = 300 ml x 0.5% = 1.5 g Table 2. Medium Composition of Nutrient Agar (NA) with the recommended proportions (Merck, 2019) Ingredients In % In Grams/300ml Peptone 0.5 *1.5 Meat extract 0.3 Agar 1.5 Distilled Water As neededarrow_forward
- (b) A Food material containing Bacillus stearothermophilus PS1518 as an indicator organism ts subjected to heat sterilization at 121 C. Calculate the time required to reduce the organism to one tenth of the original number. Fo value for the organism is 4 minutes and the decimal reduction time , D, at 116°C is 40 minutes. Assume operation is at constant temperature of 121°C. HINT Fo = -To 10 dt Where Fo = equivalent exposure time at 121°C of the actual exposure time at a variable temperature To = the reference temperature =121 C, z=10 = number ofC necessary for10fold increase in Farrow_forwardExplain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/Larrow_forwardThere are 5 TSIA media A-E beginning from the left. Describe the reactions in tubes B, C, D, E only.arrow_forward
- essential oils have been studied as potential antimicrobial agents. These naturally occuring compounds are linked to having the following type of activity in clinical trials: virucidal, septicidal, bactricidal, fungicidalarrow_forwardGiven the following media recipe, pick the terms that apply (pick as many as are applicable). 10.0 g Meat extract 10.0 g Peptone 5.0 g Sodium chloride 15.0 g 1.0 L Agar Water Once media is cool, add 6% sterile sheep's blood Defined Complex Minimal Selective Differentialarrow_forwardWhat nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include sourcearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Surgical Tech For Surgical Tech Pos CareHealth & NutritionISBN:9781337648868Author:AssociationPublisher:Cengage
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Nitrogen emissions: environmental and health hazards; Author: Sandec Eawag;https://www.youtube.com/watch?v=iYcchHZ5Ejo;License: Standard Youtube License