Microbiology with Diseases by Body System (4th Edition)
4th Edition
ISBN: 9780321918550
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 2MC
“In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals.” This statement describes which of the following?
- a. broth culture
- b. pour plate
- c. streak plate
- d. dilution plate
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Students have asked these similar questions
Which of the following is the proper technique for inoculating an agar slant with a broth culture?
A.
Stab the butt of the media with the wire loop.
B.
Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop.
C.
Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant.
D.
Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
1 ml of a patient sample was added to 999 ml of media. From this 0.1 ml was transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample.
a.
The dilution factor is 1000. The patient sample contains 600 cells/ml
b.
The dilution factor is 1000. The patient sample contains 600,000 cells/ml
c.
The dilution factor is 100. The patient sample contains 600,000 cells/ml
d.
The dilution factor is 10. The patient sample contains 6000 cells/ml
e.
It cant be determined its too many cells
Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infection
Chapter 6 Solutions
Microbiology with Diseases by Body System (4th Edition)
Ch. 6 - Why should cardiac nurses and respiratory...Ch. 6 - Cavities Gone Wild Five-year-old Daniel appears to...Ch. 6 - Why do clinical laboratory scientists keep many...Ch. 6 - Boils in the Locker Room For several weeks,...Ch. 6 - Some students transfer some gunk from a 2-week-old...Ch. 6 - Which of the following can grow in a Petri plate...Ch. 6 - In the laboratory, a sterile inoculating loop is...Ch. 6 - Superoxide dismutase _____________. a. causes...Ch. 6 - The most reactive of the four toxic forms of...Ch. 6 - Microaerophiles that grow best with a high...
Ch. 6 - Prob. 6MCCh. 6 - Organisms that preferentially may thrive in icy...Ch. 6 - Prob. 8MCCh. 6 - Which of the following terms best describes an...Ch. 6 - In a defined medium, ______________. a. the exact...Ch. 6 - Which of the following is most useful in...Ch. 6 - Which of the following methods is best for...Ch. 6 - A Coulter counter is a(n) ________________. a....Ch. 6 - Lyophilization can be described as ___________. a....Ch. 6 - Quorum sensing is _____________. a. the ability to...Ch. 6 - Prob. 1FIBCh. 6 - Prob. 2FIBCh. 6 - Prob. 3FIBCh. 6 - Prob. 4FIBCh. 6 - Prob. 5FIBCh. 6 - Fill in the Blanks 6. Cells that shrink in...Ch. 6 - Fill in the Blanks 7. Obligate ________ exist in...Ch. 6 - Prob. 8FIBCh. 6 - Fill in the Blanks 9. Microbes that reduce N2 to...Ch. 6 - Fill in the Blanks 10. A student observes a...Ch. 6 - Fill in the Blanks 11. Chemolithotrophs acquire...Ch. 6 - Prob. 1VICh. 6 - Prob. 2VICh. 6 - High temperature affects the shape of particular...Ch. 6 - Support or refute the following statement:...Ch. 6 - Explain quorum sensing, and describe how it is...Ch. 6 - Why must media, vessels, and instruments be...Ch. 6 - Why is agar used in microbiology?Ch. 6 - What is the difference between complex media and...Ch. 6 - Draw and label the four distinct phases of a...Ch. 6 - If there are 47 cells in 1 l of sewage, how many...Ch. 6 - Prob. 9SACh. 6 - Prob. 10SACh. 6 - Explain the differences among photoautotrophs,...Ch. 6 - Contrast the media described in Tables 6.3 and 6.4...Ch. 6 - How does a chemostat maintain a constant...Ch. 6 - A scientist describes an organism as a...Ch. 6 - Pasteurization is a technique that uses...Ch. 6 - Two cultures of a facultative anaerobe are grown...Ch. 6 - Some organisms require riboflavin (vitamin B2) to...Ch. 6 - A scientist inoculates a bacterium into a complex...Ch. 6 - How can regions within biofilms differ in their...Ch. 6 - A scientific article describes a bacterium as an...Ch. 6 - Prob. 8CTCh. 6 - Prob. 9CTCh. 6 - Starting with 10 bacterial cells per milliliter in...Ch. 6 - Suppose you perform a serial dilution of 0.1-ml...Ch. 6 - How might the study of biofilms benefit humans?Ch. 6 - The filamentous bacterium Beggiatoa gets its...Ch. 6 - Given that Haemophilus ducreyi is a...Ch. 6 - Examine the graph in Figure 6.3. Note that the...Ch. 6 - Prob. 16CTCh. 6 - Using the terms in Figure 6.8a, describe the...Ch. 6 - Prob. 18CTCh. 6 - Prob. 19CTCh. 6 - Prob. 20CTCh. 6 - Prob. 21CTCh. 6 - Prob. 22CTCh. 6 - Viable plate counts are used to estimate...Ch. 6 - Prob. 1CM
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- thirty-six colonies grew on nutrient agar plates when 1.0ml of a 1/10 dilution of a water sample was plated using the standard plate count methodology. How many bacteria were in the original water sample? a.4/ml b.36000/ml c.360/ml d.36/ml e.3600/mlarrow_forwardWhich of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination testarrow_forwardWhat method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreaderarrow_forward
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