The total enzyme [E t ] that was used in experiment. Introduction: Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction . All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

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Answer 1

Question

Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)

a) What are the differences between the Direct & Indirect Immunofluorescence Assays? b) What are the advantages of the Indirect Immunofluorescence Assays? c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?

Answer 2

Question

Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Expert Solution & Answer

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Answer 3

Question

Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Expert Solution & Answer

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Answer 4

Question

Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Expert Solution & Answer

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Answer 5

Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Answer 6

Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

Answer 7

Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

Answer 8

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

Answer 9

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

Answer 10

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

Answer 11

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

Answer 12

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Answer 13

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 6 - Prob. 1P Ch. 6 - Prob. 2P Ch. 6 - Prob. 3P Ch. 6 - Prob. 4P Ch. 6 - Prob. 5P Ch. 6 - Prob. 6P Ch. 6 - Prob. 7P Ch. 6 - Prob. 8P Ch. 6 - Prob. 9P Ch. 6 - Prob. 10P

Concept explainers

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To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Want to see the full answer?

Chapter 6 Solutions

Concept explainers

Videos

To determine: The value of [X]used in experiment. Introduction: Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

To explain: This enzyme is evolved to achieve catalytic perfection. Introduction: Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.

Want to see the full answer?

Chapter 6 Solutions

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with Vmax and Km of enzyme.