Biochemistry: Concepts and Connections
Biochemistry: Concepts and Connections
1st Edition
ISBN: 9780321839923
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 6, Problem 12P

The peptide hormone vasopressin is used in the regulation of saltwater balance in many vertebrates. Porcine (pig) vasopressin has the sequence
Asp-Tyr-Phe-Glu-Asn-Cys-Pro-Lys-Gly
a. Using the data in Figure 5.6 and Table 5.1, estimate the extinction coefficient e (in units of cm2/mg) for vasopressin, using radiation with λ = 280 nm.
b A solution of vasopressin is placed in a 0.5-cm-thick cuvette. Its absorbance at 280 nm is found to be 1.3. What is the concentration of vasopressin, in mg/cm3? (See Tools of Biochemistry 6A).
Chapter 6, Problem 12P, The peptide hormone vasopressin is used in the regulation of saltwater balance in many vertebrates.

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