Which technique out of norther blotting, RT-PCR and microarray can be used to study gene expression of HMG2 in yeast cells cultures in glucose and galactose? Which technique should be used to compare the expression of all the genes on chromosome 4? Introduction: Northern blotting is a technique that is used to study expression of genes by checking the concentration of RNA in a cell. RT-PCR (Real time polymerase chain reaction) involves the multiplication of cellular RNA into cDNA using reverse transcriptase and then detect its concentration from time to time or real time to determine the expression of a gene. Microarray is a technique used to study the expression of thousands of genes at a time.

Question

The shape of radishes may be long (SL/SL), oval (SL/SS), or round (SS/SS), and the color of radishes may be red (CR/CR), purple (CR/CW) or white (CW/CW). If a long, red radish plant is crossed with a round, white plant, what will be the appearance of the F1 and F2 generations?

Answer 1

Question

Chapter 6, Problem 11RTC

Summary Introduction

To analyze:

Which technique out of norther blotting, RT-PCR and microarray can be used to study gene expression of HMG2 in yeast cells cultures in glucose and galactose?

Which technique should be used to compare the expression of all the genes on chromosome 4?

Introduction:

Northern blotting is a technique that is used to study expression of genes by checking the concentration of RNA in a cell. RT-PCR (Real time polymerase chain reaction) involves the multiplication of cellular RNA into cDNA using reverse transcriptase and then detect its concentration from time to time or real time to determine the expression of a gene. Microarray is a technique used to study the expression of thousands of genes at a time.

Expert Solution & Answer

Trending nowThis is a popular solution!

See solution

Check out sample textbook solution

Students have asked these similar questions

The shape of radishes may be long (SL/SL), oval (SL/SS), or round (SS/SS), and the color of radishes may be red (CR/CR), purple (CR/CW) or white (CW/CW). If a long, red radish plant is crossed with a round, white plant, what will be the appearance of the F1 and F2 generations?

None

Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…