Anatomy and Physiology: An Integrative Approach with Connect Access Card
3rd Edition
ISBN: 9781260254440
Author: Michael McKinley, Valerie O'Loughlin, Theresa Bidle
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 5.6, Problem 21WDL
How do epithelia and connective tissue change when we age?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 5 Solutions
Anatomy and Physiology: An Integrative Approach with Connect Access Card
Ch. 5.1 - LEARNING OBJECTIVE
1. Describe the common...Ch. 5.1 - Why does an epithelium need to be highly...Ch. 5.1 - Prob. 2LOCh. 5.1 - Prob. 1WDTCh. 5.1 - Why is an epithelium considered selectively...Ch. 5.1 - Prob. 3LOCh. 5.1 - LEARNING OBJECTIVES
4. Give examples of each type...Ch. 5.1 - WHAT DO YOU THINK?
2 Which types of epithelia are...Ch. 5.1 - How does simple epithelium differ from stratified...Ch. 5.1 - What epithelial tissue lines the air sacs of the...
Ch. 5.1 - What epithelial tissue contains multiple layers of...Ch. 5.1 - LEARNING OBJECTIVES
5. Define glands.
Ch. 5.1 - Prob. 6LOCh. 5.1 - Prob. 7LOCh. 5.1 - What are the two basic parts of a multicellular...Ch. 5.1 - What are the differences between holocrine and...Ch. 5.2 - Prob. 8LOCh. 5.2 - Prob. 9LOCh. 5.2 - Prob. 10LOCh. 5.2 - Prob. 11LOCh. 5.2 - What are the basic functional differences between...Ch. 5.2 - Prob. 9WDLCh. 5.2 - Prob. 12LOCh. 5.2 - Prob. 10WDLCh. 5.2 - Prob. 13LOCh. 5.2 - Prob. 11WDLCh. 5.2 - Prob. 14LOCh. 5.2 - Prob. 3WDTCh. 5.2 - Compare loose connective tissue to dense...Ch. 5.2 - Describe the composition and location of...Ch. 5.2 - Why is blood considered a connective tissue?Ch. 5.3 - Prob. 15LOCh. 5.3 - LEARNING OBJECTIVES
16. Compare the functions of...Ch. 5.3 - Prob. 4WDTCh. 5.3 - Compare and contrast the structure of skeletal and...Ch. 5.4 - Prob. 17LOCh. 5.4 - Prob. 18LOCh. 5.4 - What is the difference between a neuron and a...Ch. 5.5 - Prob. 19LOCh. 5.5 - Prob. 20LOCh. 5.5 - Prob. 17WDLCh. 5.5 - LEARNING OBJECTIVE
21. Explain the structure and...Ch. 5.5 - LEARNING OBJECTIVE
22. Identify the locations of...Ch. 5.5 - What are the differences between the parietal and...Ch. 5.6 - Prob. 23LOCh. 5.6 - Prob. 24LOCh. 5.6 - What are the three primary germ layers, and when...Ch. 5.6 - Prob. 25LOCh. 5.6 - What is the difference between metaplasia,...Ch. 5.6 - Prob. 26LOCh. 5.6 - How do epithelia and connective tissue change when...Ch. 5 - ____ 1. Which tissue contains a calcified ground...Ch. 5 - ____ 2. Which of the following is not a...Ch. 5 - ____ 3. __________ membranes line body cavities...Ch. 5 - Do You Know the Basics?
4. Which of the...Ch. 5 - ____ 5. All of the following are characteristics...Ch. 5 - Do You Know the Basics?
6. Which connective...Ch. 5 - ____ 7. Which tissue type is formed from mesoderm?...Ch. 5 - ____ 8. Which muscle type consists of long,...Ch. 5 - ____ 9. Which epithelial tissue type lines the...Ch. 5 - ____ 10. A gland that releases its secretion by...Ch. 5 - What are some characteristics of all types of...Ch. 5 - Describe the two main criteria by which epithelia...Ch. 5 - List the epithelia type that lines (a) the lumen...Ch. 5 - Prob. 14DYBCh. 5 - Name the four types of body membranes, and cite a...Ch. 5 - What characteristics are common to all connective...Ch. 5 - What are the main structural differences between...Ch. 5 - In what regions of the body would you expect to...Ch. 5 - What are the similarities and differences between...Ch. 5 - What is the difference between neurons and glial...Ch. 5 - John is a 53-year-old construction worker who has...Ch. 5 - Your optometrist shines a light in your eye and...Ch. 5 - During a biology lab, Erin used a cotton swab to...Ch. 5 - During a biology lab, Erin used a cotton swab to...Ch. 5 - During a biology lab, Erin used a cotton swab to...Ch. 5 - During a microscopy exercise in the anatomy...Ch. 5 - Your father is suffering from a painful knee...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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