Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
11th Edition
ISBN: 9780134082318
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 55.5, Problem 2CC
WHAT IF? → In what way is the Kissimmee River project a more complete ecological restoration than the Maungatautari project (see Figure 55.17)?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 55 Solutions
Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
Ch. 55.1 - Why is the transfer of energy in an ecosystem...Ch. 55.1 - WHAT IF? You are studying nitrogen cycling on the...Ch. 55.1 - MAKE CONNECTIONS Use the second law of...Ch. 55.2 - Why is only a small portion of the solar energy...Ch. 55.2 - How can ecologists experimentally determine the...Ch. 55.2 - Prob. 3CCCh. 55.2 - MAKE CONNECTIONS Explain how nitrogen and...Ch. 55.3 - If an insect that eats plant seeds containing 100...Ch. 55.3 - Prob. 2CCCh. 55.3 - WHAT IF? Detritivores are consumers that obtain...
Ch. 55.4 - DRAW IT For each of the four biogeochemical...Ch. 55.4 - Why does deforestation of a watershed increase the...Ch. 55.4 - WHAT IF? Why is nutrient availability in a...Ch. 55.5 - Prob. 1CCCh. 55.5 - WHAT IF? In what way is the Kissimmee River...Ch. 55 - Considering the second law of thermodynamics,...Ch. 55 - Prob. 55.2CRCh. 55 - Why would runners hove a lower production...Ch. 55 - If decomposers usually grow faster and decompose...Ch. 55 - In preparing a site for surface mining and later...Ch. 55 - Which of the following organisms is incorrectly...Ch. 55 - Prob. 2TYUCh. 55 - The discipline that applies ecological principles...Ch. 55 - Level 2: Application/Analysis 4. Nitrifying...Ch. 55 - Which of the following has the greatest effect on...Ch. 55 - Prob. 6TYUCh. 55 - Which of the following would be considered an...Ch. 55 - Prob. 8TYUCh. 55 - Level 3: Synthesis/Evaluation 9. DRAW IT (a) Draw...Ch. 55 - Prob. 10TYUCh. 55 - Prob. 11TYUCh. 55 - WRITE ABOUT A THEME: ENERGY AND MATTER...Ch. 55 - Prob. 13TYU
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