The genotype of parent, F 1 , and F 2 generation. Introduction : Opium poppy plant contains two types of leaves; lacerate and normal leaves. When a cross is made between two varieties of opium plant, all the F 1 plants show the lacerate type of leaves. Two plants from F 1 generation interbred together, which results in F 2 generation with both types of leaves; lacerate and normal leaves.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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Chapter 5.2, Problem 27AQP

Summary Introduction

To determine:

The genotype of parent, F₁, and F₂ generation.

Introduction:

Opium poppy plant contains two types of leaves; lacerate and normal leaves. When a cross is made between two varieties of opium plant, all the F₁ plants show the lacerate type of leaves. Two plants from F₁ generation interbred together, which results in F₂ generation with both types of leaves; lacerate and normal leaves.

Summary Introduction

To describe:

The determination of lacerate leaves in opium poppy.

Introduction:

Opium poppy plants have lacerate and normal leaf variety. The cross between two varieties of opium plants result lacerates type of leaves in the F₁ generation. Two plants from F1 generation interbred together, which results in F₂ generation with both types of leaves; lacerate and normal leaves.

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