Laboratory Experiments in Microbiology (11th Edition)
Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 52, Problem 1Q
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To analyze:

The presence of Vibrio cholerae in water sample and still tested negative in the multiple tube technique.

Introduction:

Multiple tube technique is used to detect Coliforms in water sample which detect fecal contamination. It is done in three stages, the presumptive test, the confirmation test, and the completion stage.

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Explanation of Solution

If the water sample gives negative result for Vibrio cholerae, then it does not means that the sample does not contain this pathogen. V. choleraecan enter inside a viable and nonculturable state and then it will not form colonies on culture plates. In such a state, the cell density of V. choleraebecomes very low in water. So, if a very small amount of water sample is taken then, enough cells of this pathogen may not be present in the sample.

Conclusion

Water with high concentration of Vibrio cholera pathogenic bacterium gives negative test result in Multiple tube technique because of the presence of Vibrio cholerae in its viable and nonculturable state.

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