EBK HUMAN ANATOMY & PHYSIOLOGY
1st Edition
ISBN: 9780100659834
Author: AMERMAN
Publisher: YUZU
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Textbook Question
Chapter 5.1, Problem 2QC
Explain how the integument provides protection from potential sources of damage.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 5 Solutions
EBK HUMAN ANATOMY & PHYSIOLOGY
Ch. 5.1 - 1. What are the major structures of the skin, and...Ch. 5.1 - Explain how the integument provides protection...Ch. 5.1 - Prob. 3QCCh. 5.1 - Describe the other functions of the integument....Ch. 5.2 - 1. What are the five strata of the epidermis? How...Ch. 5.2 - Explain how a keratinocyte that begins its life in...Ch. 5.2 - In addition to keratinocytes, what three types of...Ch. 5.2 - Compare and contrast thin and thick skin.Ch. 5.3 - Which type of tissue makes up the papillary layer...Ch. 5.3 - What are the functions of the dermal papillae?
Ch. 5.3 - 3. Which type of tissue makes up the reticular...Ch. 5.3 - 4. What other structures are located in the...Ch. 5.3 - 5. How does the papillary layer of the dermis...Ch. 5.3 - What causes tension lines and flexure lines? How...Ch. 5.4 - How is melanin produced, and how does it interact...Ch. 5.4 - What are the functions of melanin?Ch. 5.4 - 3. What is carotene, and what color does it give...Ch. 5.4 - Prob. 4QCCh. 5.4 - 5. How can the oxygen content of the blood affect...Ch. 5.4 - 6. What is cyanosis, and what can it tell us...Ch. 5.5 - How do the hair shaft and hair root differ?Ch. 5.5 - How does a hair grow in length?Ch. 5.5 - Prob. 3QCCh. 5.5 - Define the following terms: nail bed, nail plate,...Ch. 5.5 - How does nail growth occur?Ch. 5.5 - Prob. 6QCCh. 5.5 - What are the other three types of sweat glands,...Ch. 5.5 - 8. How do sebaceous glands and sebum differ from...Ch. 5.6 - Prob. 1QCCh. 5.6 - Prob. 2QCCh. 5.6 - What is cancer?Ch. 5.6 - 4. How do the three types of skin cancer differ?
Ch. 5 - Explain why the skin is an organ.Ch. 5 - Which of the following correctly describes the...Ch. 5 - Which of the following is not a function of the...Ch. 5 - 4. Explain what happens to dermal blood vessels...Ch. 5 - Number the strata of thick skin epidermis from...Ch. 5 - Keratinocytes in the superficial strata of the...Ch. 5 - Mark the following statements as true or false. If...Ch. 5 - Which of the following statements is false? a....Ch. 5 - What are the functions of the dermal papillae?Ch. 5 - Epidermal ridges are created by: a. the epidermal...Ch. 5 - 11. Mark the following statements as true or...Ch. 5 - 12. Which of the following is not a function of...Ch. 5 - 13. Fill in the blanks: The portion of the hair...Ch. 5 - Nail growth occurs when: a. cells in the nail...Ch. 5 - Prob. 15CYRCh. 5 - Match each type of gland with its correct...Ch. 5 - How do sweat and sebum differ?Ch. 5 - 18. Which type of burn involves the epidermis and...Ch. 5 - 19. The type of skin tumor that involves the...Ch. 5 - Prob. 1CYUCh. 5 - Prob. 2CYUCh. 5 - The hair and nails are sometimes called accessory...Ch. 5 - 1. You are working in the emergency department...Ch. 5 - 2. After Ramon’s skin came into contact with a...Ch. 5 - 3. Which of the following is not a function of...Ch. 5 - 4. What would happen to the skin if the oil...Ch. 5 - Many antiaging skin creams contain collagen and...Ch. 5 - 6. Would a mild second-degree burn be likely to...
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