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How would a high level of HCG present in a urine sample be indicated in this agglutination test?
To determine: How would a high level of hCG hormone available in a urine sample be represented in the agglutination test.
Introduction: Monoclonal antibodies refer to the antibodies that show specificity for a single epitope alone. In monoclonal antibody preparation, a vertebrate animal, typically a mouse is immunized with an antigen several times and is killed subsequently. The B lymphocytes are combined with cancerous multiple myeloma cells using a procedure described in 1975. These myeloma cells exhibit all the properties of plasma cells, but the immunoglobulins secretion. In a suitable medium, these cells can survive forever and they will multiply indefinitely. Thus, they continuously secrete identical, monoclonal antibodies in large quantities.
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The availability of huge amounts of pure monoclonal antibodies has let the development of highly sensitive clinical lab tests. A few pregnancy tests utilize a monoclonal antibody that is produced against the human chorionic gonadotrophin (hCG) hormone. The test utilizes hCG-coated latex particles and it is exposed to an anti-hCG antibody and a urine sample. If the urine sample includes high level of hCG hormone, it will hinder attachment of antibodies to the hCG-coated latex particles and prevent their clumping (agglutination), representing pregnancy depending on the availability of hCG.
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