The method to use fluorescent tagging to track two different proteins at the same time. Introduction: The green fluorescent protein (GFP) is a protein that fluoresces and is obtained from the aquatic animal called jellyfish, having the scientific name Aequorea victoria. It is made up of 238 amino acids. It emits green fluorescent light in the presence of ultraviolet light.

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10. Consider the following enzyme and its substrate where the "+" and "-" indicate cations and anions, respectively. Explain which of the following inhibitors could inhibit this enzyme? Which type of inhibitor would it be and why? (Video 5-2) Substrate Enzyme Potential inhibitors

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Chapter 5, Problem 4Q

Summary Introduction

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The method to use fluorescent tagging to track two different proteins at the same time.

Introduction:

The green fluorescent protein (GFP) is a protein that fluoresces and is obtained from the aquatic animal called jellyfish, having the scientific name Aequorea victoria. It is made up of 238 amino acids. It emits green fluorescent light in the presence of ultraviolet light.

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10. Consider the following enzyme and its substrate where the "+" and "-" indicate cations and anions, respectively. Explain which of the following inhibitors could inhibit this enzyme? Which type of inhibitor would it be and why? (Video 5-2) Substrate Enzyme Potential inhibitors

Using Punnett Squares Punnett squares are one good way to predict the outcome of genetic crosses. Punnett squares use mathematical probability to help predict the genotype and phenotype combinations in genetic crosses. The number of possible alleles from each parent determines the number of rows and columns in the Punnett square. Independent Assortment KEY QUESTION How do alleles segregate when more than one gene is involved? Mendel wondered if the segregation of one pair of alleles affects another pair. For example, does the gene that determines the shape of a seed affect the gene for seed color? This type of experiment is known as a two-factor, or dihybrid, cross because it involves two different genes. Single-gene crosses are monohybrid crosses. Visual Reading Tool: Two-Factor Cross: F₂ The Punnett square shows the results of self-crossing the F, generation of a cross between round yellow peas and wrinkled green peas. 1. List the different genotypes in the F, generation. What is the…

CHAPTER 12 LESSON 2 Applying Mendel's Principles READING TOOL Connect to Visuals Before you read, preview Figure 12-7. Try to infer the purpose of this diagram. As you read, compare your inference to the text. After you read, revise your statement if needed or write a new one about the diagram's purpose. Take notes on the lines provided. Then view the Punnett square and answer the questions below regarding the genotypes and phenotypes. Inference: Revision: Parent 2 rryy Gametes F ry Parent 1 RRYY Gametes RY RrYy The F, generation are all RrYy. 1. What is the phenotype of parent 1?. 2. What is the genotype of parent 1? 3. What is the phenotype of parent 2? 4. What is the genotype of parent 2? 5. What is the phenotype of the F, offspring?. 6. What is the genotype of the F, offspring?. 7. What kind of cross does this figure describe? 144 Chanter 12 Introduction to Genetice Copyright Pearson Education Inc. or its affiliator. All rights reserved.