NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Chapter 5, Problem 2CT

This diagram shows the filter paper method used to evaluate the inhibitory effect of chemical agents, heavy metals, and antibiotics on bacterial growth. A culture of a test bacterium is spread uniformly over the surface of an agar plate. Small filter paper discs containing the material to be tested are then placed on the surface of the medium. A disc that has been soaked in sterile distilled water is sometimes added as a control. After incubation, a lawn (film of growth) will cover the plate, but a clear zone will surround those discs that contain an inhibitory compound. The size of the zone reflects several factors, one of which is the effectiveness of the inhibitory agent. What are two other factors that might affect the size of the zone of inhibition? What is the purpose of the control disc? If a clear area were apparent around the control disc, how would you interpret the observation?

Chapter 5, Problem 2CT, 
2. This diagram shows the filter paper method used to evaluate the inhibitory effect of chemical

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…
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