MICROBIOLOGY-ACCESS >CUSTOM<
MICROBIOLOGY-ACCESS >CUSTOM<
13th Edition
ISBN: 9780135668825
Author: Tortora
Publisher: PEARSON
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Chapter 5, Problem 10MCQ

Which culture uses the most glucose?

Use the following choices to answer questions.

  1. a. E. coli growing in glucose broth at 35°C with O2 for 5 days
  2. b. E. coli growing in glucose broth at 35°C without O2 for 5 days
  3. c. both a and b
  4. d. neither a nor b
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Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
t /g = (Log Nt – Log N0) /0.301   I introduce a loopful of Escherichia coli cells (say, 1000) into 10 mL of Nutrient Broth at 8 p.m. the night before your lab. The cells were taken from a culture plate (Nutrient Agar) held at 37°C, and inoculated into broth at the same temperature. They were held at 37°C overnight in a shaking water bath. At what time would the culture reach the Stationary Phase? Recall that doubling time under optimal conditions (these are) is 20 minutes.    A growing bacterial culture has 10,000 CFU/mL at noon and 10,000,000 CFU/mL at 6 p.m. What is the generation time under these conditions? What are your assumptions?   At midnight you inoculate 10 mL of a culture of Enterococcus with 103 cells/mL into 990 mL of the same medium, held under the same conditions as the original culture. At what time would the culture reach 107 cells/mL? Assume exponential growth over the period. Assume that g=half an hour. Note: We worked a different variant of this problem in…
THIS IS A MULTIPART QUESTION, PLEASE ANSWER ALL Using the image: 1. How would you categorize this medium? (Living/nonliving, Synthetic/complex, Rich/minimal) Briefly explain your responses. 2. Name the ingredient(s) that match the following nutrients: a. Source(s) of carbon b. Source(s) of nitrogen c. At least one source of minerals d. At least one source of growth factors, if any 3. What about this medium would change if agar were added as an ingredient? 4. Would you expect this medium to be differential, selective, or both? Explain your response.

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