To determine:
The reason to inoculate urinary specimen in MacConkey.
Introduction:
Bacteria are biological cells that are prokaryotic in nature. They are the first living form to occur on the Earth and are found in most of the habitats. The length of bacteria varies a lot and they can be rod, spherical, or spiral in shape.
Explanation of Solution
MacConkey is a differential solid media used for the isolation of enteric gram-negative bacteria only. Its agar contains bile-salt neutral red-lactose. The purpose of bile salt and crystal violet in MacConkey is to inhibit the growth of fastidious gram-negative bacteria and gram-positive bacteria. The bacteria that harm the urinary tract and are responsible for urinary tract infections are mostly enteric gram-negative bacteria. So, for inoculation of urinary specimen, MacConkey agar is used.
The bacteria responsible for urinary infections are enteric gram-negative. So, urinary specimen are inoculated in MacConkey agar.
Want to see more full solutions like this?
Chapter 49 Solutions
Laboratory Experiments in Microbiology (11th Edition)
- which bacteria is Pleomorphicrod or clubshaped,gram positive, motile and passes the oxidase test? which bacteria is Coccobacillary,gram negative, non motile and negative oxidase test? which bacteria is Rods withparallel sidesand roundedends, gram negative, non motile and negative oxidase test? which bacteria is Coccoid assingles or inpairs, gram positive, non motile and negative oxidase test?arrow_forwardIs the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a “needle” used to inoculate?arrow_forward1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! Thanksarrow_forward
- Why is water tested for coliform bacteria rather than for pathogenic bacteria which may be present?arrow_forwardWhy are the streak plates incubated at 7°C when selecting for psychrotrophic bacteria?arrow_forwardWhy is washing with Hank’s balanced salt solution with 0.5% antibiotic-antimycotic done for Splenocyte Preparation?arrow_forward