BROOKER BIO 3-HOLE PUNCH W/CONNECT BUND
BROOKER BIO 3-HOLE PUNCH W/CONNECT BUND
5th Edition
ISBN: 9781264065615
Author: BROOKER
Publisher: MCGRAW-HILL LEARNING SOLN.(CC)
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Chapter 48.8, Problem 3EQ
Summary Introduction

To propose: A hypothesis that explains the presence of high levels of the Pco2 in the newborn’s blood.

Introduction: The circulation of various nutrients and gases to all the body tissues takes place through the blood. The levels of Pco2 depict the amount of carbon dioxide present in an organism. The blood transfers the gases to and from the body tissues.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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