The tests that is necessary further to identify red colony producing bacteria on MacConkey agar. Introduction: MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

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Answer 1

Question

Chapter 48, Problem 1Q

Summary Introduction

To analyze:

The tests that is necessary further to identify red colony producing bacteria on MacConkey agar.

Introduction:

MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

Expert Solution & Answer

Explanation of Solution

The gram negative rods that is oxidase-negative and that which ferments lactose can be subjected to check for the presence of Hydrogen sulphide production. If Hydrogen sulphide test is positive the bacterium can be a Citrobacter. If it is negative to Hydrogen sulphide test then Indole test can be performed. If it is positive to Indole test it is Escherichia and if it is negative mobility test can be done. If there is mobility then it can be Enterobacter and if there is no mobility then it is Klebsiella.

Conclusion

Thus the above tests can be performed to confirm the identity of the bacterium.

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1. In vivo testing provides valuable insight into a drug’s kinetics. Assessing drug kinetics following multiple different routes of administration provides greater insight than just a single route of administration alone. The following data was collected in 250 g rats following bolus iv, oral (po), and intraperitoneal (ip) administration.Using this data and set of graphs, determine: (a) k, C0, V, and AUC* for the bolus iv data (b) k, ka, B1, and AUC* for the po data (c) k, ka, B1, and AUC* for the ip data (d) relative bioavailability for po vs ip, Fpo/Fip (e) absolute po bioavailability, (f)Fpo absolute ip bioavailability, Fip MAKE SURE ANSWERS HAVE UNITS if appropriate. SHOW ALL WORK, including equation used, variables used and each step to your solution.

2. Drug quantification from plasma is commonly performed by using techniques such as HPLC or LC/MS. However, these methods do have limitations, and investigators may choose to use a radiolabeled analog of a drug instead. Radioligands are molecules that contain radioactive isotopes, commonly 3H or 14C. This technique allows investigators to quantify drug concentration from radiation measurements. The following measurements were made in 250 g rats following oral administration of 18.2 µCi of a 14C-labeled drug of interest: Time (min) Plasma Radiation Levels (µCi/L) 0 0.0 2 9.7 4 19.2 7 25.3 9 37.8 12 39.6 14 45.8 17 48.8 20 52.0 25 56.4 30 59.2 35 60.1 40 61.1 45 62.1 50 62.8 60 63.1 70 62.1 80 60.1 90 57.3 100 55.5 110 53.7 120 52.2 150 48.0 180 45.0 240 39.0 Note that a µCi is a measure of the amount of radioactivity and hence is a measure of the amount of drug present. Given that the oral bioavailability of this drug is known to be essentially 100%, estimate the following from this…

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Answer 2

Question

Chapter 48, Problem 1Q

Summary Introduction

To analyze:

The tests that is necessary further to identify red colony producing bacteria on MacConkey agar.

Introduction:

MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

Expert Solution & Answer

Explanation of Solution

The gram negative rods that is oxidase-negative and that which ferments lactose can be subjected to check for the presence of Hydrogen sulphide production. If Hydrogen sulphide test is positive the bacterium can be a Citrobacter. If it is negative to Hydrogen sulphide test then Indole test can be performed. If it is positive to Indole test it is Escherichia and if it is negative mobility test can be done. If there is mobility then it can be Enterobacter and if there is no mobility then it is Klebsiella.

Conclusion

Thus the above tests can be performed to confirm the identity of the bacterium.

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Answer 3

Question

Chapter 48, Problem 1Q

Summary Introduction

To analyze:

The tests that is necessary further to identify red colony producing bacteria on MacConkey agar.

Introduction:

MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

Expert Solution & Answer

Explanation of Solution

The gram negative rods that is oxidase-negative and that which ferments lactose can be subjected to check for the presence of Hydrogen sulphide production. If Hydrogen sulphide test is positive the bacterium can be a Citrobacter. If it is negative to Hydrogen sulphide test then Indole test can be performed. If it is positive to Indole test it is Escherichia and if it is negative mobility test can be done. If there is mobility then it can be Enterobacter and if there is no mobility then it is Klebsiella.

Conclusion

Thus the above tests can be performed to confirm the identity of the bacterium.

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Answer 4

Question

Chapter 48, Problem 1Q

Summary Introduction

To analyze:

The tests that is necessary further to identify red colony producing bacteria on MacConkey agar.

Introduction:

MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

Expert Solution & Answer

Explanation of Solution

The gram negative rods that is oxidase-negative and that which ferments lactose can be subjected to check for the presence of Hydrogen sulphide production. If Hydrogen sulphide test is positive the bacterium can be a Citrobacter. If it is negative to Hydrogen sulphide test then Indole test can be performed. If it is positive to Indole test it is Escherichia and if it is negative mobility test can be done. If there is mobility then it can be Enterobacter and if there is no mobility then it is Klebsiella.

Conclusion

Thus the above tests can be performed to confirm the identity of the bacterium.

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Answer 5

Chapter 48, Problem 1Q

Summary Introduction

To analyze:

The tests that is necessary further to identify red colony producing bacteria on MacConkey agar.

Introduction:

MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

Expert Solution & Answer

Explanation of Solution

The gram negative rods that is oxidase-negative and that which ferments lactose can be subjected to check for the presence of Hydrogen sulphide production. If Hydrogen sulphide test is positive the bacterium can be a Citrobacter. If it is negative to Hydrogen sulphide test then Indole test can be performed. If it is positive to Indole test it is Escherichia and if it is negative mobility test can be done. If there is mobility then it can be Enterobacter and if there is no mobility then it is Klebsiella.

Conclusion

Thus the above tests can be performed to confirm the identity of the bacterium.

Answer 6

Chapter 48, Problem 1Q

Summary Introduction

To analyze:

The tests that is necessary further to identify red colony producing bacteria on MacConkey agar.

Introduction:

MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

Expert Solution & Answer

Explanation of Solution

The gram negative rods that is oxidase-negative and that which ferments lactose can be subjected to check for the presence of Hydrogen sulphide production. If Hydrogen sulphide test is positive the bacterium can be a Citrobacter. If it is negative to Hydrogen sulphide test then Indole test can be performed. If it is positive to Indole test it is Escherichia and if it is negative mobility test can be done. If there is mobility then it can be Enterobacter and if there is no mobility then it is Klebsiella.

Conclusion

Thus the above tests can be performed to confirm the identity of the bacterium.

Answer 7

Chapter 48, Problem 1Q

Summary Introduction

To analyze:

The tests that is necessary further to identify red colony producing bacteria on MacConkey agar.

Introduction:

MacConkey agar stands as a differential media of lactose and non-lactose fermenters by producing red colonies for the former and colourless colonies for the latter respectively. Further certain tests can be undergone to identify the organism.

Answer 8

Expert Solution & Answer

Explanation of Solution

The gram negative rods that is oxidase-negative and that which ferments lactose can be subjected to check for the presence of Hydrogen sulphide production. If Hydrogen sulphide test is positive the bacterium can be a Citrobacter. If it is negative to Hydrogen sulphide test then Indole test can be performed. If it is positive to Indole test it is Escherichia and if it is negative mobility test can be done. If there is mobility then it can be Enterobacter and if there is no mobility then it is Klebsiella.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Conclusion

Thus the above tests can be performed to confirm the identity of the bacterium.

Answer 12

Ch. 48 - Prob. 1Q Ch. 48 - Differentiate among a coliform, an enteric that is...Ch. 48 - Is MacConkey agar a selective medium?...Ch. 48 - Is PEAâ€”blood agar a selective medium?...Ch. 48 - Prob. 1CT Ch. 48 - Prob. 2CT Ch. 48 - Prob. 1CA

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