BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
12th Edition
ISBN: 9781264058167
Author: Raven
Publisher: MCG CUSTOM
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Textbook Question
Chapter 47, Problem 3S
Explain how bacteria, archaea, protists, and many phyla of invertebrates can survive without respiratory organs.
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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Chapter 47 Solutions
BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
Ch. 47.1 - Prob. 1LOCh. 47.1 - Prob. 2LOCh. 47.1 - Compare evolutionary strategies for maximizing gas...Ch. 47.2 - Prob. 1LOCh. 47.2 - Prob. 2LOCh. 47.3 - Prob. 1LOCh. 47.3 - Prob. 2LOCh. 47.3 - Describe the breathing cycle of birds.Ch. 47.4 - Prob. 1LOCh. 47.4 - Prob. 2LO
Ch. 47.4 - Prob. 3LOCh. 47.5 - Describe the structure of hemoglobin.Ch. 47.5 - Predict hemoglobins oxygen affinity in various...Ch. 47.5 - Prob. 3LOCh. 47 - Prob. 1DACh. 47 - Prob. 2DACh. 47 - Prob. 3DACh. 47 - Prob. 4DACh. 47 - For a given PO2, how does oxyhemoglobin saturation...Ch. 47 - Prob. 1IQCh. 47 - Prob. 2IQCh. 47 - Prob. 1UCh. 47 - Increased efficiency of gas exchange in...Ch. 47 - Prob. 3UCh. 47 - Prob. 4UCh. 47 - Ficks Law of Diffusion states the rate of...Ch. 47 - Prob. 6UCh. 47 - Prob. 7UCh. 47 - Prob. 8UCh. 47 - When you take a deep breath, your stomach moves...Ch. 47 - Marine mammals are able to hold their breath for...Ch. 47 - Countercurrent flow systems do not occur in lungs...Ch. 47 - Respiratory organs of invertebrates and...Ch. 47 - Prob. 5ACh. 47 - During exercise more oxygen is delivered to the...Ch. 47 - Compare the operation and efficiency of fish gills...Ch. 47 - What happens when, during exercise, the oxygen...Ch. 47 - Explain how bacteria, archaea, protists, and many...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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