BIOLOGY CONNECT ACCESS CARD
12th Edition
ISBN: 9781264037452
Author: Raven
Publisher: MCG
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Chapter 4.7, Problem 3LO
Summary Introduction
To classify: The elements of extracellular matrix in animal cells.
Introduction: The extracellular matrix is a three-dimensional, non-cellular part of a tissue, and it is a network of extracellular macromolecules, such as enzymes, collagen, and glycoproteins, that confer structural and biochemical support to the surrounding cells.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 4 Solutions
BIOLOGY CONNECT ACCESS CARD
Ch. 4.1 - Discuss the cell theory.Ch. 4.1 - Describe the factors that limit cell size.Ch. 4.1 - Prob. 3LOCh. 4.2 - Describe the organization of prokaryotic cells.Ch. 4.2 - Prob. 2LOCh. 4.3 - Prob. 1LOCh. 4.3 - Prob. 2LOCh. 4.3 - Prob. 3LOCh. 4.4 - Prob. 1LOCh. 4.4 - Contrast the different functions of internal...
Ch. 4.4 - Prob. 3LOCh. 4.5 - Describe the structure of mitochondria and...Ch. 4.5 - Compare the function of mitochondria and...Ch. 4.5 - Prob. 3LOCh. 4.6 - Contrast the structure and function of different...Ch. 4.6 - Prob. 2LOCh. 4.7 - Prob. 1LOCh. 4.7 - Prob. 2LOCh. 4.7 - Prob. 3LOCh. 4.8 - Prob. 1LOCh. 4.8 - Prob. 2LOCh. 4 - Prob. 1IQCh. 4 - Prob. 2IQCh. 4 - Prob. 3IQCh. 4 - Which of the following statements is NOT part of...Ch. 4 - All cells have all of the following except a....Ch. 4 - Eukaryotic cells are more complex than prokaryotic...Ch. 4 - Which of the following are differences between...Ch. 4 - The cytoskeleton includes a. microtubules made of...Ch. 4 - The smooth endoplasmic reticulum is a. involved in...Ch. 4 - Plasmodesmata in plants and gap junctions in...Ch. 4 - The most important factor that limits the size of...Ch. 4 - All eukaryotic cells possess each of the following...Ch. 4 - Adherens junctions, which contain cadherin, are...Ch. 4 - Different motor proteins like kinesin and myosin...Ch. 4 - The protein sorting pathway involves the following...Ch. 4 - Chloroplasts and mitochondria have many common...Ch. 4 - Prob. 7ACh. 4 - The smooth endoplasmic reticulum is the site of...Ch. 4 - Use the information provided in table 4.3 to...Ch. 4 - Prob. 3SCh. 4 - The protist Giardia intestinalis is the organism...
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