1 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781265486297
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 47, Problem 2U
Increased efficiency of gas exchange in vertebrates has been brought about by all of the following mechanisms except
a. cutaneous respiration.
b. unidirectional air flow.
c. crosscurrent blood flow.
d. cartilaginous rings in the trachea.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 47 Solutions
1 SEM ACC W/RAVEN CARDED
Ch. 47.1 - Prob. 1LOCh. 47.1 - Prob. 2LOCh. 47.1 - Compare evolutionary strategies for maximizing gas...Ch. 47.2 - Prob. 1LOCh. 47.2 - Prob. 2LOCh. 47.3 - Prob. 1LOCh. 47.3 - Prob. 2LOCh. 47.3 - Describe the breathing cycle of birds.Ch. 47.4 - Prob. 1LOCh. 47.4 - Prob. 2LO
Ch. 47.4 - Prob. 3LOCh. 47.5 - Describe the structure of hemoglobin.Ch. 47.5 - Predict hemoglobins oxygen affinity in various...Ch. 47.5 - Prob. 3LOCh. 47 - Prob. 1DACh. 47 - Prob. 2DACh. 47 - Prob. 3DACh. 47 - Prob. 4DACh. 47 - For a given PO2, how does oxyhemoglobin saturation...Ch. 47 - Prob. 1IQCh. 47 - Prob. 2IQCh. 47 - Prob. 1UCh. 47 - Increased efficiency of gas exchange in...Ch. 47 - Prob. 3UCh. 47 - Prob. 4UCh. 47 - Ficks Law of Diffusion states the rate of...Ch. 47 - Prob. 6UCh. 47 - Prob. 7UCh. 47 - Prob. 8UCh. 47 - When you take a deep breath, your stomach moves...Ch. 47 - Marine mammals are able to hold their breath for...Ch. 47 - Countercurrent flow systems do not occur in lungs...Ch. 47 - Respiratory organs of invertebrates and...Ch. 47 - Prob. 5ACh. 47 - During exercise more oxygen is delivered to the...Ch. 47 - Compare the operation and efficiency of fish gills...Ch. 47 - What happens when, during exercise, the oxygen...Ch. 47 - Explain how bacteria, archaea, protists, and many...
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