BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
12th Edition
ISBN: 9781264058167
Author: Raven
Publisher: MCG CUSTOM
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Chapter 46.2, Problem 2LO
Summary Introduction
To explain: The role of the mouth in the process of digestion.
Introduction: The
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Students have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 46 Solutions
BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
Ch. 46.1 - Prob. 1LOCh. 46.1 - Prob. 2LOCh. 46.1 - Prob. 3LOCh. 46.2 - Prob. 1LOCh. 46.2 - Prob. 2LOCh. 46.3 - Prob. 1LOCh. 46.3 - Prob. 2LOCh. 46.4 - Prob. 1LOCh. 46.4 - Explain how absorbed nutrients move into the blood...Ch. 46.5 - Prob. 1LO
Ch. 46.5 - Prob. 2LOCh. 46.5 - Prob. 3LOCh. 46.6 - Prob. 1LOCh. 46.6 - Prob. 2LOCh. 46.7 - Prob. 1LOCh. 46.7 - Prob. 2LOCh. 46.7 - Prob. 3LOCh. 46.8 - Prob. 1LOCh. 46.8 - Describe how rumination works.Ch. 46.8 - Prob. 3LOCh. 46 - Prob. 1DACh. 46 - If you constructed a phylogeny using molecular...Ch. 46 - Prob. 1IQCh. 46 - Prob. 2IQCh. 46 - Prob. 3IQCh. 46 - Prob. 4IQCh. 46 - How is the digestion of fats different from that...Ch. 46 - Although the stomach is normally thought of as the...Ch. 46 - After being absorbed through the intestinal...Ch. 46 - Which of these pairings is incorrect? a. Fat...Ch. 46 - Intestinal microorganisms aid digestion and...Ch. 46 - Prob. 6UCh. 46 - Which of the following represents the action of...Ch. 46 - Prob. 1ACh. 46 - The primary function of the large intestine is to...Ch. 46 - Inactive forms of some molecules are secreted a....Ch. 46 - Prob. 4ACh. 46 - Many birds possess crops, although few mammals do....Ch. 46 - Suppose that you wanted to develop a new treatment...Ch. 46 - How could a drop in plasma proteins and a decrease...Ch. 46 - Unlike many cases of convergence (see section...Ch. 46 - Prob. 5S
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