Pearson eText for Laboratory Experiments in Microbiology -- Instant Access (Pearson+)
12th Edition
ISBN: 9780137546503
Author: Ted Johnson, Christine Case
Publisher: PEARSON+
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Textbook Question
Chapter 44, Problem 1Q
Was the test performed in this exercise a direct or an indirect ELISA test?
Expert Solution & Answer
Summary Introduction
To fill:
Indirect ELISA.
Introduction:
Immunoassays are tests done for the diagnosis of diseases by identifying the presence of antigen or a particular antibody from a patient's sample. Antigen-Antibody interactions form the basis of immunoassays wherein interactions like agglutinations, precipitation is seen. Immunoassays using enzyme labels are termed as ELISA-Enzyme Linked ImmunoSorbent Assay.
Answer to Problem 1Q
The test performed in this exercise is indirect ELISA.
Explanation of Solution
In Indirect ELISA first the antigen is added to the well and then the primary antibody is added. After the buffer wash, enzyme-labeled secondary antibody is added which binds to the Antigen-primary antibody complex. Finally, the substrate corresponding to the enzyme is added, which forms a color emitting enzyme-substrate complex. The color change indicates that appropriate Antibody is present in the patient's sample.
In this exercise, initially, S.entericaTyphimurium (antigen) is added, and the patient's serum (i.e primary antibody) is added followed by the addition of alkaline phosphatase labeled with secondary antibody. Finally, the alkaline phosphatase substrate (BCIP/NBT) is added in order to give color if there is an interaction between the antigen and the primary antibody.
Hence the test followed in the exercise is Indirect ELISA.
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Transcription and Translation
1. What is the main function of transcription and translation? (2
marks)
2. How is transcription different in eukaryotic and prokaryotic
cells? (2 marks)
3. Explain the difference between pre-mRNA and post-transcript
mRNA. (2 marks)
4. What is the function of the following: (4 marks)
i. the cap
ii. spliceosome
iii. Poly A tail
iv. termination sequence
5. What are advantages to the wobble feature of the genetic
code? (2 marks)
6. Explain the difference between the: (3 marks)
i. A site & P site
ii. codon & anticodon
iii. gene expression and gene regulation
7. Explain how the stop codon allows for termination. (1 mark)
8. In your own words, summarize the process of translation. (2
marks)
In this activity you will research performance enhancers that affect the
endocrine system or nervous system. You will submit a 1 page paper on one
performance enhancer of your choice. Be sure to include:
the specific reason for use
the alleged results on improving performance
how it works
how it affect homeostasis and improves performance
any side-effects of this substance
Chapter 44 Solutions
Pearson eText for Laboratory Experiments in Microbiology -- Instant Access (Pearson+)
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