Campbell Biology (11th Edition)
11th Edition
ISBN: 9780134093413
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 43, Problem 8TYU
Summary Introduction
To draw: The antibody-linking protein complex that could trigger endocytosis by a macrophage.
Introduction: An antigen is a large molecule. It may be made up of proteins or polysaccharides. A single antigen has several binding sites that are called epitopes. Each antibody binds to a specific epitope of an antigen. An antibody is a Y-shaped protein molecule that consists of four polypeptide chains. There are two identical heavy chains and two identical light chains connected by a disulfide bond. Antibody binds to the antigen receptor of pathogens like bacteria or virus.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 43 Solutions
Campbell Biology (11th Edition)
Ch. 43.1 - Pus is both a sign of infection and an indicator...Ch. 43.1 - MAKE CONNECTIONS How do the molecules that...Ch. 43.1 - Prob. 3CCCh. 43.2 - Prob. 1CCCh. 43.2 - Prob. 2CCCh. 43.2 - WHAT IF? If both copies of a light-chain gene and...Ch. 43.3 - Prob. 1CCCh. 43.3 - Treatment of antibodies with a particular protease...Ch. 43.3 - Prob. 3CCCh. 43.4 - Prob. 1CC
Ch. 43.4 - Prob. 2CCCh. 43.4 - WHAT IF? How would a macrophage deficiency likely...Ch. 43 - In what ways does innate immunity protect the...Ch. 43 - Why is the adaptive immune response to an initial...Ch. 43 - Is immunological memory after a natural infection...Ch. 43 - Prob. 43.4CRCh. 43 - Prob. 1TYUCh. 43 - An epitope associates with which part of an...Ch. 43 - Prob. 3TYUCh. 43 - Level 2: Application/Analysis 4. Which of the...Ch. 43 - Prob. 5TYUCh. 43 - Prob. 6TYUCh. 43 - Prob. 7TYUCh. 43 - Prob. 8TYUCh. 43 - MAKE CONNECTIONS Contrast clonal selection with...Ch. 43 - EVOLUTION CONNECTION Describe one invertebrate...Ch. 43 - Prob. 11TYUCh. 43 - Prob. 12TYUCh. 43 - SYNTHESIZE YOUR KNOWLEDGE This photo shows a child...
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