The term “MHC” denotes “Major Histocompatibility Complex” molecule. This molecule is the host protein that presents the “antigen fragment” on the surface of the cell. It is essential for “T cells” to make out the foreign antigen. In the same manner, the “MHC molecules” identify the cells from another person as foreign and attack them. This is called immune rejection. These MHC molecules are identical between identical twins, so there will not be immune rejection if an organ is transplanted between identical twins.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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Chapter 43, Problem 5TYU

Summary Introduction

Introduction: The term “MHC” denotes “Major Histocompatibility Complex” molecule. This molecule is the host protein that presents the “antigen fragment” on the surface of the cell. It is essential for “T cells” to make out the foreign antigen. In the same manner, the “MHC molecules” identify the cells from another person as foreign and attack them. This is called immune rejection. These MHC molecules are identical between identical twins, so there will not be immune rejection if an organ is transplanted between identical twins.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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