Biology
Biology
10th Edition
ISBN: 9780321794260
Author: Audesirk, Teresa/ Audesirk
Publisher: Pearson College Div
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Chapter 41.2, Problem 1CYL
Summary Introduction

To describe:

The human male and female reproductive tracts including gonads and accessory structures along with the function of each structure.

Introduction:

The reproductive system is the organ which produces the gametes and aids the production of new offspring. In male the reproductive organ is testis which produces sperm. In females reproductive organ is ovary that produces egg.

Summary Introduction

To explain:

The hormonal interactions control in reproduction in men and women.

Introduction:

Male hormone testosterone is important for the development of male secondary sexual characteristics. The interstitial cell present between the seminiferous tubules secretes the male sex hormone called androgen. Androgen is important for the production of testosterone. Female sex hormones estrogen and progesterone are important for the maturation and development of female gamete.

Summary Introduction

To describe:

The spermatogenesis and oogenesis along with their location and timing. Also explain the important features of sperm and egg and how fertilization occurs.

Introduction:

Spermatogenesis and oogenesis are the processes of production and development of male and female gametes respectively.

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Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
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